Author: Jim Rutkowski

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AUTHOR

Jim Rutkowski

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Advanced Platelet-Rich Fibrin: A New Concept for Cell-Based Tissue Engineering by Means of Inflammatory Cells

2014

Choukroun's platelet-rich fibrin (PRF) is obtained from blood without adding anticoagulants. In this study, protocols for standard platelet-rich fibrin (S-PRF) (2700 rpm, 12 minutes) and advanced platelet-rich fibrin (A-PRF) (1500 rpm, 14 minutes) were compared to establish by histological cell detection and histomorphometrical measurement of cell distribution the effects of the centrifugal force (speed and time) on the distribution of cells relevant for wound healing and tissue regeneration. Immunohistochemistry for monocytes, T and B -lymphocytes, neutrophilic granulocytes, CD34-positive stem cells, and platelets was performed on clots produced from four different human donors. Platelets …

AdultBlood PlateletsPathologymedicine.medical_specialtyBone RegenerationErythrocytesTime FactorsAdolescentNeutrophilsT-LymphocytesAntigens CD34CentrifugationInflammationCell SeparationMonocytesFibrinYoung AdultTissue engineeringmedicineHumansRegenerationPlateletB-LymphocytesFibrinTissue Engineeringbiologybusiness.industryMacrophagesStem CellsCell DifferentiationMiddle AgedImmunohistochemistrydigestive system diseasesPlatelet-rich fibrinBlood Buffy Coatbiology.proteinOral Surgerymedicine.symptombusinessCell basedJournal of Oral Implantology

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PEPhysical Sciences and Engineering
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LSLife Sciences
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