serrano gene and NaCl perception

Gustatory information is considered to be important for animals to control behavior when they seek for food or partners. 60 members of gustatory receptor (Gr) genes have been identified in Drosophila.. However little is now about mechanisms of taste perception and transduction in response to stimuli. In order to identify new genes involved in gustation, we performed a genetic screen using Gal4-UAS system based on expression of the reporter gene in chemosensory organs and gustatory defects in larvae and adults. We identified the serrano (sano) gene, which encodes a 778 aa protein with a leucine zipper domain, a putative transmembrane domain, and putative bipartite nuclear localization signal…

Multiple HAT transporters regulate dopaminergic transmission and sleep in Drosophila

Small fatty acids perception: effect during fly life

serrano gene and NaCl perception in Drosophila

Résumé de poster présenté au congrès Neurofly à Manchester en Septembre 2010.; International audience

Drosophila and humans share similar mechanisms of insulin secretion

Drosophila and humans share similar mechanisms of insulin secretion

Direct Sensing of Nutrients via a LAT1-like Transporter in Drosophila Insulin-Producing Cells

Summary Dietary leucine has been suspected to play an important role in insulin release, a hormone that controls satiety and metabolism. The mechanism by which insulin-producing cells (IPCs) sense leucine and regulate insulin secretion is still poorly understood. In Drosophila, insulin-like peptides (DILP2 and DILP5) are produced by brain IPCs and are released in the hemolymph after leucine ingestion. Using Ca2+-imaging and ex vivo cultured larval brains, we demonstrate that IPCs can directly sense extracellular leucine levels via minidiscs (MND), a leucine transporter. MND knockdown in IPCs abolished leucine-dependent changes, including loss of DILP2 and DILP5 in IPC bodies, consistent wit…

Organisateurs de la 18e rencontre du Club de neurobiologie des invertébrés

The Amino Acid Transporter JhI-21 Coevolves with Glutamate Receptors, Impacts NMJ Physiology, and Influences Locomotor Activity in Drosophila Larvae

AbstractChanges in synaptic physiology underlie neuronal network plasticity and behavioral phenomena, which are adjusted during development. The Drosophila larval glutamatergic neuromuscular junction (NMJ) represents a powerful synaptic model to investigate factors impacting these processes. Amino acids such as glutamate have been shown to regulate Drosophila NMJ physiology by modulating the clustering of postsynaptic glutamate receptors and thereby regulating the strength of signal transmission from the motor neuron to the muscle cell. To identify amino acid transporters impacting glutmatergic signal transmission, we used Evolutionary Rate Covariation (ERC), a recently developed bioinforma…

Cyclic AMP-dependent and independent stimulations of ovarian steroidogenesis by brain factors in the blowfly, Phormia regina.

0303-7207 doi: DOI: 10.1016/S0303-7207(00)00312-9; The involvement of cyclic-AMP (cAMP) as a potential second messenger in the neurohormonal control of ovarian steroidogenesis was investigated in the adult female blowfly Phormia regina. Individual measurements of ovarian cAMP concentrations and of ovarian biosynthesis of ecdysteroids, stimulated after a protein meal, demonstrated that steroidogenesis is preceded by a peak of cAMP in the ovaries. In vitro, ovarian steroidogenesis was stimulated by cell-permeable analogues of cAMP and by forskolin. Crude brain extracts were also able to elicit a rise of cAMP in the ovaries in vitro and the secretion of ecdysteroids into the medium: such extra…

Chemoreceptors and lead to opposite behaviors along drosophila life cycle

Chemoreceptors and lead to opposite behaviors along drosophila life cycle. 16. european neurobiology of drosophila conference

An inhibitory sex pheromone tastes bitter for Drosophila males

1932-6203 (Electronic) Journal Article; Sexual behavior requires animals to distinguish between the sexes and to respond appropriately to each of them. In Drosophila melanogaster, as in many insects, cuticular hydrocarbons are thought to be involved in sex recognition and in mating behavior, but there is no direct neuronal evidence of their pheromonal effect. Using behavioral and electrophysiological measures of responses to natural and synthetic compounds, we show that Z-7-tricosene, a Drosophila male cuticular hydrocarbon, acts as a sex pheromone and inhibits male-male courtship. These data provide the first direct demonstration that an insect cuticular hydrocarbon is detected as a sex ph…

Role of MINIDISCS, a SLC7A amino-acid transporter, on glutamatergic activity in Drosophila melanogaster mushroom bodies.

Dropping lile flies

Dropping lile flies. 17. rencontre du Club de neurobiologie des invertébrés

Direct sensing of leucine by insulin-producing neurons

Direct sensing of leucine by insulin-producing neurons. MoucheRhône

Impact of heterodimeric amino acid transporters on metabolism: Input of a genetic model

Comment le dialogue moléculaire entre bactéries et neurones change le comportement de l’hôte infecté

National audience; Les eucaryotes vivent dans un environnement contaminé par des microorganismes. Il n'est donc pas surprenant qu'ils aient forgé, au fil du temps, des relations extrêmement complexes et intimes entre eux. Les eucaryotes sont capables de percevoir la présence de bactéries et d’adapter leur réponse immunitaire, leur état physiologique ou même leur comportement en conséquence. Nombreuses sont les études qui ont démontré que les bactéries peuvent interagir avec le système nerveux eucaryote, soit au bénéfice du microbe qui modifie le comportement de l'hôte, soit au bénéfice de l'hôte qui adapte son comportement à l'infection. Dans la plupart des cas, cependa…