Methylone determination in oral fluid using microextraction by packed sorbent coupled to ion mobility spectrometry

Abstract A methodology based on microextraction by packed sorbent (MEPS) followed by ion mobility spectrometry (IMS) has been developed for the simple and automated determination of methylone in oral fluids. MEPS was carried out using a 100 µL eVol® XR hand-held automated analytical syringe filled with octadecyl silica sorbent. The effect of main experimental parameters of MEPS such as sample pH and ionic strength, number of aspirating/dispensing cycles, and elution solvent was studied to obtain a quantitative extraction of methylone. Oral fluid samples were spiked with methylone at different concentration levels from 50 to 400 µg L−1 and analyzed by the proposed MEPS-IMS procedure, giving …