0000000001267061

AUTHOR

L. Galiana-balaguer

showing 2 related works from this author

Determination of -Ascorbic Acid in Lycopersicon Fruits by Capillary Zone Electrophoresis

2001

This study shows an improved method for the determination of L-ascorbic acid (l-AA) in fruits of Lycopersicon by capillary zone electrophoresis (CZE). Two backgrounds electrolytes (BGEs) have been tested: (i) 400 mM borate at pH 8.0 and 1 x 10(-2)% hexadimethrine bromide, for the separation of Eulycopersicon subgenus species; and (ii) as in BGE(i) but supplemented with 20% (v/v) acetonitrile, for the separation of species of the Eriopersicon subgenus. The present procedures were compared with two routine methods-enzymatic assay and potentiometric titration with 2,6-dichlorophenol-indophenol. While these routine methods presented some difficulties in quantifying l-AA in several Lycopersicon …

Detection limitHexadimethrine bromideChromatographybiologyPotentiometric titrationBiophysicsElectrophoresis Capillaryfood and beveragesImproved methodAscorbic AcidCell BiologyAscorbic acidbiology.organism_classificationBiochemistryLycopersiconInternal qualitychemistry.chemical_compoundCapillary electrophoresisSolanum lycopersicumchemistryFruitPotentiometryMolecular BiologyAnalytical Biochemistry
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Simultaneous quantification of the main organic acids and carbohydrates involved in tomato flavour using capillary zone electrophoresis

2002

A capillary zone electrophoresis (CZE) procedure for the simultaneous determination of the major organic acids (oxalate, malate and citrate) and carbohydrates (fructose, glucose and sucrose) in Lycopersicon fruits is reported. Comparison of this method with routine HPLC methods indicates that the CZE method offers several attractive features (speed, resolution, sensitivity and cost) which significantly improve the determination of these compounds. Detection limits were better than 1.6 µg ml−1 for organic acids and from 13 to 24 µg ml−1 for carbohydrates; repeatabilities were better than 2.1% for migration times and between 1.4 and 7.3% for peak areas. The proposed protocol is very useful to…

Detection limitNutrition and DieteticsSucroseChromatographyResolution (mass spectrometry)FructoseHigh-performance liquid chromatographyOxalatechemistry.chemical_compoundCapillary electrophoresischemistryAgronomy and Crop ScienceQuantitative analysis (chemistry)Food ScienceBiotechnologyJournal of the Science of Food and Agriculture
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