0000000001276268
AUTHOR
Sandra Munti��n
Additional file 1 of Co-administration of human MSC overexpressing HIF-1�� increases human CD34+ cell engraftment in vivo
Additional file 1. Additional methods.
Additional file 3 of Co-administration of human MSC overexpressing HIF-1�� increases human CD34+ cell engraftment in vivo
Additional file 3. Figure S2. Immunophenotypic analysis of MSC WT and HIF-MSC. Histogram of the different surface molecules analyzed by flow cytometry. MSC WT: MSC wild type; HIF-MSC: MSC overexpressing HIF-1��
Additional file 4 of Co-administration of human MSC overexpressing HIF-1�� increases human CD34+ cell engraftment in vivo
Additional file 4. Figure S3. Analysis of human hematopoietic engraftment in spleen. Human hematopoietic engraftment was evaluated by flow cytometry after xenotrasplantation in NOD/SCID mice. The percentage of human CD45+ cells (donor chimerism) was analyzed in total spleen samples 4 weeks after transplantation. Mice were transplanted intravenously with CD34+ cells alone or co-transplanted with MSC WT or HIF-MSC, that were administered in the right femur. Data are represented as mean of 8 experiments for mice transplanted with CD34+ cells alone, 13 experiments for mice co-transplanted with CD34+ cells and WT MSC and 15 experiments for mice transplanted with CD34+ cells and HIF-MSC. p:n.s me…
Additional file 2 of Co-administration of human MSC overexpressing HIF-1�� increases human CD34+ cell engraftment in vivo
Additional file 2. Figure S1. Transduction efficiency of MSC. Histogram of a representative sample, with transfection efficiency >90%. MSC WT: MSC wild type; HIF-MSC: MSC overexpressing HIF-1��; GFP: Green Fluorescent Protein