0000000001295907
AUTHOR
Alberto Paradela
Multicentric study of the effect of pre-analytical variables in the quality of plasma samples stored in biobanks using different complementary proteomic methods.
12 páginas, 7 figuras.-- Jesús Mateos ... et al.
In-Depth Proteomic Characterization of Classical and Non-Classical Monocyte Subsets
Monocytes are bone marrow-derived leukocytes that are part of the innate immune system. Monocytes are divided into three subsets: classical, intermediate and non-classical, which can be differentiated by their expression of some surface antigens, mainly CD14 and CD16. These cells are key players in the inflammation process underlying the mechanism of many diseases. Thus, the molecular characterization of these cells may provide very useful information for understanding their biology in health and disease. We performed a multicentric proteomic study with pure classical and non-classical populations derived from 12 healthy donors. The robust workflow used provided reproducible results among t…
Blockade of the trans-sulfuration pathway in acute pancreatitis due to nitration of cystathionine β-synthase
© 2019 Published by Elsevier B.V.
A multicentric study to evaluate the use of relative retention times in targeted proteomics.
Despite the maturity reached by targeted proteomic strategies, reliable and standardized protocols are urgently needed to enhance reproducibility among different laboratories and analytical platforms, facilitating a more widespread use in biomedical research. To achieve this goal, the use of dimensionless relative retention times (iRT), defined on the basis of peptide standard retention times (RT), has lately emerged as a powerful tool. The robustness, reproducibility and utility of this strategy were examined for the first time in a multicentric setting, involving 28 laboratories that included 24 of the Spanish network of proteomics laboratories (ProteoRed-ISCIII). According to the results…
Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis
6 p.-2 fig.-2 tab.