0000000001302200
AUTHOR
Lucas Del Castillo Agudo
Phenotype traits associated with different alleles at the RPS5 locus in Saccharomyces cerevisiae
The RPS5 gene has been characterised through its ability to reduce invertase production by the SUC5 gene. In this paper we show that RPS5 acts by maintaining low levels of SUC5 mRNA. We also show that RPS5 acts on the SUC1 and SUC4 genes but not on SUC2 and SUC3, which are members of the SUC family. RPS5 also shows a pleiotropic effect on the amount of mitochondrial cytochromes.
Mixed-ligand copper(ii)–sulfonamide complexes: effect of the sulfonamide derivative on DNA binding, DNA cleavage, genotoxicity and anticancer activity
Four ternary complexes, [Cu(L1)2(bipy)] (1) [HL1 = N-(6-chlorobenzo[d]thiazol-2-yl)-4-methylbenzenesulfonamide], [Cu(L2)2(bipy)] (2) [HL2 = N-(benzo[d]thiazol-2-yl)-4-methylbenzenesulfonamide], [Cu(L3)2(bipy)]·1/2H2O (3) [HL3 = N-(5,6-dimethylbenzo[d]thiazol-2-yl)-4-methylbenzenesulfonamide] and [Cu(L4)2(bipy)] (4) [HL4 = N-(5,6-dimethylbenzo[d]thiazol-2-yl)benzenesulfonamide], were prepared and then characterized by X-ray crystallography, spectroscopy and magnetic measurements. Whereas the molecular structure of 1 and 2 consists of a discrete monomeric copper(II) species with a distorted square planar geometry, that of 3 and 4 consists of two independent molecules. In 3, both molecules pre…
Strong protective action of Copper(II) N-substituted sulfonamide complexes against reactive oxygen species.
Copper(II) complexes of N -benzothiazolsulfonamides, [Cu( N -2-(5,6-dimethylbenzothiazole)toluenesulfonamidate) 2 (dmso) 2 ] ( 1 ), [Cu( N -2-(6-chlorobenzothiazole)benzenesulfonamidate) 2 (dmso) 2 ] ( 2 ) and [Cu( N -2-(6-chlorobenzothiazole)toluenesulfonamidate) 2 (dmso) 2 ] ( 3 ) with interesting protective properties against superoxide radicals have been prepared. The compounds have been characterized by X-ray diffraction and their chemical properties have been studied by spectroscopic methods. The crystal structure of 1 shows that the copper(II) is surrounded by two benzothiazole N atoms from the sulfonamide ligands and two O atoms from the dimethylsulfoxide molecules in a square plana…
Differential expression of SUC genes: A question of bases
Non-coding nucleotide sequences located 5' upstream of the transcriptional start site play an essential role in gene expression as they contain binding sites for transcription and regulatory factors. The yeast SUC gene family is a useful model to study the influence that nucleotide exchanges within the promoter regions have on their expression, since (i) these genes, regulated by glucose repression, are differentially transcribed (invertase activity produced by distinct SUC genes may show variations of about 10-fold); and (ii) promoter sequences of SUC3, SUC4, SUC5 and SUC7 are more than 99% homologous, showing only six base exchanges among all of them. Comparison of these nucleotide exchan…
Lipid content of Saccharomyces cerevisiae strains with different degrees of ethanol tolerance
We analysed the fatty acid and sterol compositions of various Saccharomyces cerevisiae strains with ethanol tolerance varying from 4% to 12% (v/v) ethanol and at different concentrations of ethanol. The results we obtained agree with the existence of a relationship between membrane fluidity and ethanol tolerance but they do not support a direct role of unsaturated fatty acids in this tolerance. On the other hand, they support the importance of ergosterol in this phenomenon.
Differential expression of the invertase-encoding SUC genes in Saccharomyces cerevisiae
Invertase (INV) is encoded in Saccharomyces cerevisiae by a family of genes, comprising SUC1-SUC5 and SUC7. Production of INV is highly variable, dependent on the strain and SUC gene present in the cell. The differences in INV production derive from the structure of the genes or are dependent on the genetic background of the strain. Centromeric plasmids (based on YCp50) carrying one of the SUC genes (except SUC7) were introduced into a strain (SEY2101) lacking SUC genes. The INV produced by the transformants was dependent on the individual SUC genes, and correlated with INV mRNA levels. Plasmids in which SUC2 had been placed under control of promoters from the other SUC genes, were used to …
Selection and long-term cultures of high-yielding Digitalis obscura plants: RAPD markers for analysis of genetic stability
Fifteen wild-growing plants of Digitalis obscura collected in three differents regions were characterized according to their capacities to biosynthesize cardenolides and to proliferate in vitro. Great genotype-dependent variabilities were found in both parameters. Selected genotypes were differentiated using randomly amplified polymorphic DNA (RAPD) markers. Once an elite genotype with high-yielding properties was isolated and micropropagated during 2 years, identity of the donor plant and the regenerants was confirmed by RAPD analysis.
Comparison of Protective Effects against Reactive Oxygen Species of Mononuclear and Dinuclear Cu(II) Complexes with N-Substituted Benzothiazolesulfonamides
Copper(II) complexes of N-benzothiazolesulfonamides (HL1=N-2-(4-methylphenylsulfamoyl)-6-nitro-benzothiazole, HL2=N-2-(phenylsulfamoyl)-6-chloro-benzothiazole, and HL3=N-2-(4-methylphenylsulfamoyl)-6-chloro-benzothiazole) with ammonia have been synthesized and characterized. The crystal structures of the [Cu(L1)2(NH3)2].2MeOH, [Cu(L2)2(NH3)2], and [Cu(L3)2(NH3)2] compounds have been determined. Compounds and present a distorted square planar geometry. In both compounds the metal ion is coordinated by two benzothiazole N atoms from two sulfonamidate anions and two NH3 molecules. Complex is distorted square-pyramidal. The Cu(II) ion is linked to the benzothiazole N and sulfonamidate O atoms o…
Isolation and characterization of extracellular vesicles in Candida albicans
Background : The occurrence of systemic infections due to C. albicans has increased especially in critically ill patients. In fungal infections, secretory mechanisms are key events for disease establishment. Recent findings demonstrate that fungal organisms release many molecular components to the extracellular space in extracellular vesicles. Aims: We develop a method to obtain exosomes from yeast cultures of the Candida albicans . Methods : Yeast strains used in this work were C. albicans SC5314, C. parapsilosis (ATCC 22019) and C. krusei (ATCC 6258). Yeasts were grown at 37.º in liquid YPD medium. The cell cultures were centrifuged and the supernatant filtered through sterile nitrocellul…
Differentiation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis by specific PCR amplification of the RPS0 intron
Although Candida parapsilosis is the most prevalent among the 3 species of the *psilosis group, studies applying DNA-based diagnostic techniques with isolates previously identified as C. parapsilosis have revealed that both C. orthopsilosis and C. metapsilosis account for 0-10% of all these isolates, depending on the geographical area. Differences in the degrees of antifungal susceptibility and virulence have been found, so a more precise identification is required. In a first approach, we reidentified 38 randomly chosen clinical isolates, previously identified as C. parapsilosis, using the RPO2 (CA2) RAPD marker. Among them, we reclassified 4 as C. metapsilosis and 5 as C. orthopsilosis. W…
Manganese(II) complexes of scorpiand-like azamacrocycles as MnSOD mimics.
Mn(II) complexes of scorpiand-type azamacrocycles constituted by a tretrazapyridinophane core appended with an ethylamino tail including 2- or 4-quinoline functionalities show very appealing in vitro SOD activity. The observed behaviour is related to structural and electrochemical parameters.
Isolation of aCandida albicans gene, tightly linked toURA3, coding for a putative transcription factor that suppresses aSaccharomyces cerevisiaeaft1 mutation
A pathogen such as C. albicans needs an ef®cient mechanism of iron uptake in an iron- restricted environment such as is the human body. A ferric-reductase activity regulated by iron and copper, and analogous to that in S. cerevisiae, has been described in C. albicans. We have developed an in-plate protocol for the isolation of clones that complement an aft1 mutation in S. cerevisiae that makes cells dependent on iron for growth. After transformation of S. cerevisiae aft1 with a C. albicans library, we have selected clones that grow in conditions of iron de®ciency and share an identical plasmid, pIRO1, with a 4500 bp insert containing the URA3 gene and an ORF (IRO1) responsible for the suppr…
CCDC 923557: Experimental Crystal Structure Determination
Related Article: Marta Gonzalez-Alvarez,Alejandro Pascual-Alvarez,Lucas del Castillo Agudo,Alfonso Castineiras,Malva Liu-Gonzalez,Joaquin Borras,Gloria Alzuet-Pina|2013|Dalton Trans.|42|10244|doi:10.1039/C3DT50416F
CCDC 923555: Experimental Crystal Structure Determination
Related Article: Marta Gonzalez-Alvarez,Alejandro Pascual-Alvarez,Lucas del Castillo Agudo,Alfonso Castineiras,Malva Liu-Gonzalez,Joaquin Borras,Gloria Alzuet-Pina|2013|Dalton Trans.|42|10244|doi:10.1039/C3DT50416F
CCDC 923556: Experimental Crystal Structure Determination
Related Article: Marta Gonzalez-Alvarez,Alejandro Pascual-Alvarez,Lucas del Castillo Agudo,Alfonso Castineiras,Malva Liu-Gonzalez,Joaquin Borras,Gloria Alzuet-Pina|2013|Dalton Trans.|42|10244|doi:10.1039/C3DT50416F
CCDC 923558: Experimental Crystal Structure Determination
Related Article: Marta Gonzalez-Alvarez,Alejandro Pascual-Alvarez,Lucas del Castillo Agudo,Alfonso Castineiras,Malva Liu-Gonzalez,Joaquin Borras,Gloria Alzuet-Pina|2013|Dalton Trans.|42|10244|doi:10.1039/C3DT50416F