0000000001305223
AUTHOR
Tanja M. Lahtinen
Development of functionalized SYBR green II related cyanine dyes for viral RNA detection
Fluorescent probes for sensing nucleic acids have found widespread use in the field of cell and molecular biology. However, probes combined with potential for post-synthetic conjugation, e.g. for intra-endosomal measurements of RNA, are unavailable. Herein we developed cyanine dyes that can be conjugated to viral capsid or other targets. First, we solved the crystal structure of SYBR Green II. The structural elucidation of this commonly used RNA probe provided the basis for synthesizing similar molecules with much desired function for post-synthetic conjugation. To address this need, cyanine dyes were prepared using an alternative synthesis protocol. All studied compounds showed considerabl…
Systematic study of SYBR green chromophore reveals major improvement with one heteroatom difference
Five nucleic acid binding cyanine dyes were synthesized and their photophysical properties were evaluated. Changing a single heteroatom in the chromophore causes major differences both in brightness and photostability between the dyes. With such alteration, the brightness of the chromophore increased two-fold compared to the one found in SYBR Green I. peerReviewed
CCDC 1482405: Experimental Crystal Structure Determination
Related Article: Ville K. Saarnio, Kirsi Salorinne, Visa P. Ruokolainen, Jesper R. Nilsson, Tiia-Riikka Tero, Sami Oikarinen, L. Marcus Wilhelmsson, Tanja M. Lahtinen, Varpu S. Marjomäki|2020|Dyes Pigm.|177|108282|doi:10.1016/j.dyepig.2020.108282
CCDC 2194827: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844
CCDC 2194825: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844
CCDC 2194823: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844
CCDC 1482404: Experimental Crystal Structure Determination
Related Article: Ville K. Saarnio, Kirsi Salorinne, Visa P. Ruokolainen, Jesper R. Nilsson, Tiia-Riikka Tero, Sami Oikarinen, L. Marcus Wilhelmsson, Tanja M. Lahtinen, Varpu S. Marjomäki|2020|Dyes Pigm.|177|108282|doi:10.1016/j.dyepig.2020.108282
CCDC 2194826: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844
CCDC 2194824: Experimental Crystal Structure Determination
Related Article: Johanna M. Alaranta, Khai-Nghi Truong, María Francisca Matus, Sami A. Malola, Kari T. Rissanen, Sailee S. Shroff, Varpu S. Marjomäki, Hannu J. Häkkinen, Tanja M. Lahtinen|2022|Dyes Pigm.|208|110844|doi:10.1016/j.dyepig.2022.110844