Author: Anton Podjava

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AUTHOR

Anton Podjava

showing 12 related works from this author

Zwitterionic Sulfonates as m/z Shift Reagents for 5-Methylcytosine Detection in Deoxyribonucleic Acids (DNA) Using Flow Injection Analysis and Electr…

2015

5-Methylcytosine (5-MC) is an important epigenetic modification of DNA. Abnormally high concentrations of this substance appear because of the hypermethylation of cytosine. Therefore, the measurement of the quantity of this compound in mammals is of great importance. Recently, we reported that several imidazolium-based zwitterionic sulfonates form complexes with 5-MC in solution, which can be studied by electrospray ionisation mass spectrometry (ESI-MS). It is shown in this paper that such an association can be utilised for the detection of 5-MC in a DNA sample using high-throughput a flow injection analysis ESI-MS method. A variety of the sulfonate zwitterions have been tested as m/ z shi…

Detection limitFlow injection analysisSpectrometry Mass Electrospray IonizationElectrosprayChromatographyChemistryElectrospray ionizationAnalytical chemistryReproducibility of ResultsDNAGeneral MedicineDNA MethylationTandem mass spectrometryMass spectrometryAtomic and Molecular Physics and OpticsSample preparation in mass spectrometryFlow Injection Analysis5-MethylcytosineAnimalsCattleA-DNASulfonic AcidsSpectroscopyEuropean Journal of Mass Spectrometry

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Synthesis and sorptive properties of molecularly imprinted polymer for simultaneous isolation of catecholamines and their metabolites from biological…

2021

Simultaneous quantitative analysis of catecholamines (CAs) and their respective acid metabolites in biological fluids can become a powerful tool for diagnosis and treatment of various diseases. However, selective sample preparation procedure is necessary to isolate the aforementioned analytes from the matrix. Therefore, we propose a synthetic strategy for the preparation of a molecularly imprinted sorbent with binding sites for both CAs and their respective acid metabolites. The synthesis combines noncovalent and semicovalent imprinting methods. Methylenebisacrylamide, 4-vynilbenzyl-N,N,N-trimethylammonium (3,4-dimethoxyphenyl)acetate, and 3-phenylpropylacrylate were used as the cross-linke…

Chromatography010405 organic chemistryChemistryfungi010401 analytical chemistryClinical BiochemistryMolecularly imprinted polymerfood and beveragesPharmaceutical ScienceIsolation (microbiology)01 natural sciencesBiochemistry0104 chemical sciencesAnalytical ChemistryBiological fluidsQuantitative analysis (chemistry)

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Study of Chromatographic Properties of Catecholamines and their Acidic Metabolites Using Novel Molecularly Imprinted Polymers as Stationary Phases

2021

Quantitative analysis of catecholamines and their acidic metabolites can provide vital information for diagnosis and treatment of various diseases (such as adrenal tumors). However, complicated purification protocols are usually required to isolate the analytes. Molecularly imprinted polymers (MIPs) have attracted considerable attention as a method of selectively separating desired compounds from their matrix. Therefore, we propose a synthetic procedure for new molecularly imprinted sorbents that possess selective binding sites for both catecholamines and their acidic metabolites. The new polymer utilizes non-covalent and semi-covalent imprinting methods. The binding properties of MIPs were…

ChromatographyMechanics of MaterialsChemistryStationary phaseMechanical EngineeringMolecularly imprinted polymerGeneral Materials ScienceKey Engineering Materials

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Electrospray Ionization Mass Spectrometry of Non-Covalent Complexes Formed between N-Alkylimidazolium-Containing Zwitterionic Sulfonates and Protonat…

2015

This paper describes non-covalent complexes between zwitterionic 3-(1-alkyl-3 N-imidazolio)-propane-1-sulfonates and different amines. Electrospray ionization (ESI) mass spectrometry and collision-induced dissociation were used to measure the stability of such complexes in solution and in the gas phase. Generally, zwitterionic sulfonates formed more abundant complexes with protonated 5-methylcytosine (5-MCH) than with aliphatic amines. The results show that the association constants and half-dissociation threshold energies of these complexes nonlinearly depend on the alkyl chain length of the zwitterion. It is shown that the complexes with the lowest stability exist in acetonitrile solutio…

chemistry.chemical_classificationCollision-induced dissociationHydrogen bondElectrospray ionizationProtonationGeneral MedicineMass spectrometryAtomic and Molecular Physics and OpticsDissociation (chemistry)chemistry.chemical_compoundchemistryComputational chemistryZwitterionSpectroscopyAlkylEuropean Journal of Mass Spectrometry

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Evaluation of furanocoumarins from seeds of the wild parsnip (Pastinaca sativa L. s.l.).

2018

Abstract Although the wild parsnip (Pastinaca sativa L. s.l.) fruits are known to contain linear and angular furanocoumarins, the individual components of the seeds have not been fully identified and quantitated, and, in the case of immature seeds, reported. In view of this, the main furanocoumarin compounds were extracted using pyridine, and were isolated using semi-preparative high-performance liquid chromatography. The structural elucidation of isolated compounds was done based on detailed spectral analysis conducted by liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI/MS), 1H and 13C NMR and, where possible, by gas chromatography–mass spectrometry (GC–MS). The quan…

IsopimpinellinSpectrometry Mass Electrospray IonizationClinical BiochemistryWild parsnip030226 pharmacology & pharmacy01 natural sciencesBiochemistryBergaptenGas Chromatography-Mass SpectrometryAnalytical Chemistry03 medical and health scienceschemistry.chemical_compoundFuranocoumarin0302 clinical medicinefoodLimit of DetectionFurocoumarinsmedicinePastinacaPastinacaChromatography High Pressure LiquidChromatographyApiaceaebiologyImperatorinMethoxsalen010401 analytical chemistryReproducibility of ResultsCell BiologyGeneral Medicinebiology.organism_classificationfood.food0104 chemical scienceschemistrySeedsLinear Modelsmedicine.drugJournal of chromatography. B, Analytical technologies in the biomedical and life sciences

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Crystal and Molecular Structure and Stability of Isoniazid Cocrystals with Selected Carboxylic Acids

2013

Reaction of isoniazid with benzoic acid, sebacic acid, suberic acid, and cinnamic acid results in formation of cocrystals. Two polymorphs of isoniazid–suberic acid and two polymorphs of isoniazid–cinnamic acid cocrystals were isolated. Crystal structure analysis shows the presence of a pyridine–carboxylic acid synthon in the studied cocrystals. The hydrazide group of isoniazid participates in N–H···O and N–H···N hydrogen bond formation, producing different supramolecular synthons. The stability study of isoniazid cocrystals has been performed over a 22 week period. A comparison of melting points of isoniazid–dicarboxylic acid 2:1 cocrystals shows the decrease of melting point with an increa…

Sebacic acidHydrogen bondSynthonGeneral Chemistrybacterial infections and mycosesCondensed Matter PhysicsHydrazideCinnamic acidchemistry.chemical_compoundchemistryPolymer chemistryOrganic chemistryGeneral Materials ScienceSolubilitySuberic acidBenzoic acidCrystal Growth & Design

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Positive and negative electrospray ionization-collision-induced dissociation of sulfur- containing zwitterionic liquids.

2011

The mass spectrometric properties of several (1,2-dimethyl-1 H-imidazol-3-ium-3-yl)-alkane-1-sulfonates (alkane=ethyl, propyl and butyl) are investigated in this study. These substances, named zwitterionic liquids (ZILs), were synthesized using classical transformations and analyzed in positive and negative electrospray ionization mode using collision-induced dissociation (0–50 eV). We have also performed regioselective deuterium labeling of the alkyl chain of 3-(1,2-dimethyl-1 H-imidazol-3-ium-3-yl)-propane-1-sulfonates. Thus, the mass spectra of isotopically-labeled compounds were used for the confirmation of fragmentation pathways of ZILs. Briefly, the data obtained in this study show t…

chemistry.chemical_classificationElectrosprayCollision-induced dissociationElectrospray ionizationInorganic chemistryGeneral MedicinePhotochemistryAtomic and Molecular Physics and OpticsDissociation (chemistry)chemistry.chemical_compoundSulfonatechemistryFragmentation (mass spectrometry)Mass spectrumSpectroscopyAlkylEuropean journal of mass spectrometry (Chichester, England)

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