0000000001306187
AUTHOR
Miguel Perez-aso
Annexin A2-Mediated Plasminogen Activation in Endothelial Cells Contributes to the Proangiogenic Effect of Adenosine A2A Receptors
Adenosine A2A receptor mediates the promotion of wound healing and revascularization of injured tissue, in healthy and animals with impaired wound healing, through a mechanism depending upon tissue plasminogen activator (tPA), a component of the fibrinolytic system. In order to evaluate the contribution of plasmin generation in the proangiogenic effect of adenosine A2A receptor activation, we determined the expression and secretion of t-PA, urokinase plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1) and annexin A2 by human dermal microvascular endothelial cells stimulated by the selective agonist CGS-21680. The plasmin generation was assayed through an enzymatic assay a…
Additional file 1: Figure S1. of Apremilast, a novel phosphodiesterase 4 (PDE4) inhibitor, regulates inflammation through multiple cAMP downstream effectors
Adenosine A2A receptor (A2AR) activation and apremilast do not additively increase responsive element binding protein (cAMP). Raw 264.7 cells were incubated with cumulative concentrations of apremilast (6 nM to 1 μM), apremilast + CGS21680 1μM 15 minutes before apremilast, or cumulative concentrations of CGS21680 alone (6 nM to 1 μM), followed by treatment with lipopolysaccharide (LPS) 1 μM for 20 minutes. Then, intracellular cAMP levels were measured as described under “Materials and methods”. Data represent means ± standard error of the mean of at least three independent experiments. (TIFF 170 kb)
Additional file 2: Figure S2. of Apremilast, a novel phosphodiesterase 4 (PDE4) inhibitor, regulates inflammation through multiple cAMP downstream effectors
Permanent transduction of shRNA for protein kinase (PKA) dramatically decreases PKA protein expression in the Raw 264.7 cell line, as determined by western blotting. (TIFF 268 kb)