0000000001306866
AUTHOR
Erik Westberg
Additional file 1: Table S1. of Genetic variation of naturally growing olive trees in Israel: from abandoned groves to feral and wild?
SSR markers used, their expected size range, repeated motives and number of alleles found in naturally growing olive populations. Raw microsatellite data is available and enclosed as Additional file 2: Table S2. (PDF 188 kb)
Environmental effects on molecular and phenotypic variation in populations of Eruca sativa across a steep climatic gradient
Abstract In Israel Eruca sativa has a geographically narrow distribution across a steep climatic gradient that ranges from mesic Mediterranean to hot desert environments. These conditions offer an opportunity to study the influence of the environment on intraspecific genetic variation. For this, we combined an analysis of neutral genetic markers with a phenotypic evaluation in common-garden experiments, and environmental characterization of populations that included climatic and edaphic parameters, as well as geographic distribution. A Bayesian clustering of individuals from nine representative populations based on amplified fragment length polymorphism (AFLP) divided the populations into a…
Phylogeography ofSyringa josikaea(Oleaceae): Early Pleistocene divergence from East Asian relatives and survival in small populations in the Carpathians
Tertiary relict plant species of Europe have had a large distribution range before the Pleistocene but today are confined to small refugial areas. Syringa josikaea of the largely East Asian genus Syringa is a shrub of temperate forests in the Carpathians, restricted to altogether 25 small populations in two disjunct areas, the Apuseni Mountains (Romania) and the Ukrainian Carpathians. Miocene and Pleistocene fossil remains indicate the long-term presence of the species in Central Europe; hence S. josikaea has been considered a Tertiary relict. We aimed at clarifying the historical biogeography of S. josikaea by estimating the divergence time between S. josikaea and its Asian relatives, and …
Additional file 5: Figure S2. of Genetic variation of naturally growing olive trees in Israel: from abandoned groves to feral and wild?
â K values for the different Ks were calculated according to Evanno et al. [56], showing that K = 3 is the optimal K for the Structure analysis. (PDF 69 kb)
Additional file 4: Figure S1. of Genetic variation of naturally growing olive trees in Israel: from abandoned groves to feral and wild?
Number of private alleles per locus in combinations of populations. A to D present values for the combination of two to five populations (treating scions and suckers of old olive trees as populations). (PDF 217 kb)
Additional file 6: Figure S3. of Genetic variation of naturally growing olive trees in Israel: from abandoned groves to feral and wild?
Location of populations of naturally growing olives analyzed in this study and of groves of cultivated old olive trees sampled in our previous study (Barazani et al. [33]). (PDF 79 kb)
Genetic variation of naturally growing olive trees in Israel: from abandoned groves to feral and wild?
Background Naturally growing populations of olive trees are found in the Mediterranean garrigue and maquis in Israel. Here, we used the Simple Sequence Repeat (SSR) genetic marker technique to investigate whether these represent wild var. sylvestris. Leaf samples were collected from a total of 205 trees at six sites of naturally growing olive populations in Israel. The genetic analysis included a multi-locus lineage (MLL) analysis, Rousset’s genetic distances, Fst values, private alleles, other diversity values and a Structure analysis. The analyses also included scions and suckers of old cultivated olive trees, for which the dominance of one clone in scions (MLL1) and a second in suckers (…
Ecological differentiation and reproductive isolation of two closely related sympatric species of Oenanthe (Apiaceae)
Oenanthe conioides is a lower Elbe endemic plant species growing in the freshwater tidal zone around Hamburg (Germany). Its closest relative Oenanthe aquatica is widely distributed in Eurasia and grows in calm and shallow freshwater. The two species differ in habitat requirements but are otherwise sympatrically distributed, suggesting that ecological divergence has to be maintained in the face of gene flow. In the present study, we investigated ecological differentiation and reproductive isolation in these two species. An amplified fragment length polymorphism analysis found clear genetic differentiation between the two species implying reproductive isolation. A reciprocal transplantation e…
Genetic evidence for divergent selection onOenanthe conioidesandOe. aquatica(Apiaceae), a candidate case for sympatric speciation
The opportunity for habitat shift in sympatry is thought to be an important factor in sympatric speciation by facilitating assortative mating and offering opportunities for divergent selection. Oenanthe conioides (Apiaceae) is a narrow endemic from the lower Elbe river area (Germany) where it is restricted to areas experiencing fresh water tides inundating the plants twice a day. The species was shown to have originated from Oe. aquatica which is widely distributed in Europe and grows in still or slowly flowing fresh water. Reciprocal transplant experiments have previously shown that in both habitats the non-native species is less fit than the native, and several phenotypic traits have been…
Soil seed bank and among-years genetic diversity in arid populations of Eruca sativa Miller (Brassicaceae)
Abstract Populations of Eruca sativa from arid environments exhibit greater physiological dormancy than those that originated in more favorable ones. We tested the possible relative contribution of the soil seed bank (SSB) to the aboveground cohorts in desert and semiarid populations. Seedlings that emerged from the SSB under controlled irrigation were distinguished from those that emerged after exposure of the remaining ungerminated SSB to gibberellic acid as constituting non-dormant and dormant SSBs, respectively. On average, the total SSB was significantly larger in the semiarid than in the desert population. In both sites, the aboveground and SSB cohorts exhibited closely similar geneti…
A comparative analysis of genetic variation in rootstocks and scions of old olive trees – a window into the history of olive cultivation practices and past genetic variation
Background Past clonal propagation of olive trees is intimately linked to grafting. However, evidence on grafting in ancient trees is scarce, and not much is known about the source of plant material used for rootstocks. Here, the Simple Sequence Repeat (SSR) marker technique was used to study genetic diversity of rootstocks and scions in ancient olive trees from the Levant and its implications for past cultivation of olives. Leaf samples were collected from tree canopies (scions) and shoots growing from the trunk base (suckers). A total of 310 trees were sampled in 32 groves and analyzed with 14 SSR markers. Results In 82.7% of the trees in which both scion and suckers could be genotyped, t…
Phylogeographic evidence for the postglacial colonization of the North and Baltic Sea coasts from inland glacial refugia by Triglochin maritima L.
Abstract We investigated the geographical distribution of genetic variation in 67 individuals of Triglochin maritima from 38 localities across Europe using AFLP markers. Analysis of genetic variation resulted in the recognition of two major genetic groups. Apart from few geographical outliers, these are distributed (1) along the Atlantic coasts of Portugal, Spain and France and (2) in the North Sea area, the Baltic Sea area, at central European inland localities, the northern Adriatic Sea coast and the Mediterranean coast of southwest France. Considering possible range shifts of T. maritima in reaction to Quaternary climatic changes as deduced from the present-day northern temperature limit…
The identity and geographical distribution of Jacobaea vulgaris subsp. gotlandica , supposedly endemic to Gotland and Öland (Sweden)-the importance of multiple intraspecific samples
The identity of Jacobaea vulgaris Gaertn. subsp. gotlandica (Neum.) B. Nord., hitherto considered an endemic to the Swedish islands Oland and Gotland, was investigated using nrITS and trnT-trnL intergenic spacer sequences and a broad sample of species and accessions particularly of J. vulgaris. We found that J. vulgaris subsp. gotlandica is a distinct evolutionary lineage more widespread in Europe than previously thought and may be a southeastern European steppe floristic element with outliers on the large Baltic islands. Our entirely unexpected findings underline the need of multiple intraspecific sampling in species-level phylogenetic studies.
Testing the potential significance of different scion/rootstock genotype combinations on the ecology of old cultivated olive trees in the southeast Mediterranean area
Background A previous multi-locus lineage (MLL) analysis of SSR-microsatellite data of old olive trees in the southeast Mediterranean area had shown the predominance of the Souri cultivar (MLL1) among grafted trees. The MLL analysis had also identified an MLL (MLL7) that was more common among rootstocks than other MLLs. We here present a comparison of the MLL combinations MLL1 (scion)/MLL7 (rootstock) and MLL1/MLL1 in order to investigate the possible influence of rootstock on scion phenotype. Results A linear regression analysis demonstrated that the abundance of MLL1/MLL7 trees decreases and of MLL1/MLL1 trees increases along a gradient of increasing aridity. Hypothesizing that grafting o…
The influence of sea currents, past disruption of gene flow and species biology on the phylogeographical structure of coastal flowering plants
Aim We investigate the geographical genetic structure of two coastal plant species, Cakile maritima Scop. (Brassicaceae) and Eryngium maritimum L. (Apiaceae), through three sea straits and along one continuous stretch of coast using amplified fragment length polymorphisms (AFLPs). The two species have a similar ecology in that they grow in sandy habitats, but differ in life-form (annual vs. perennial) and dispersability of seeds by sea water as inferred from floating experiments. The sea straits differ in their geological history and their modern current systems. The primary goal of our study was to test the hypothesis that sea straits have an influence on the geographical patterns of genet…
MOESM3 of Testing the potential significance of different scion/rootstock genotype combinations on the ecology of old cultivated olive trees in the southeast Mediterranean area
Additional file 3. Results of the model-selection procedure.
Terrestrial growth and marine dispersal? Comparative phylogeography of five coastal plant species at a European scale
The phylogeography of five flowering plant species (Cakile maritima, Eryngium maritimum, Salsola kali, Halimione portulacoides, Crithmum maritimum) widespread along the European coasts was investigated across their entireEuropean range using AFLP evidence. Both similarities and dissimilarities were found. All species contain a distinct Black Sea/Aegean Sea cluster, and all except E. maritimum contain a distinct Adriatic Sea cluster or group of genetically very similar clusters. All species except Cr. maritimum contain a distinct Atlantic Ocean/North Sea/Baltic Sea cluster clearly separate from the Mediterranean material. In Ca. maritima a distinct Baltic Sea subcluster was found. In the wes…
MOESM1 of Testing the potential significance of different scion/rootstock genotype combinations on the ecology of old cultivated olive trees in the southeast Mediterranean area
Additional file 1: Figure S1. Box plot comparisons of phenotypic traits in old olive trees of the MLL1/MLL7 (GG1) and MLL1/MLL1 (GG2) scion/sucker combinations. Traits included the oil content in the fruits, waste water content obtained in the oil extraction process, four oil quality characteristics and three morphological properties of the fruits and stones.
Additional file 3: Table S3. of Genetic variation of naturally growing olive trees in Israel: from abandoned groves to feral and wild?
Number of olive trees assigned to different multi-locus lineages (MLLs). (XLSX 18 kb)
Additional file 2: Table S2. of Genetic variation of naturally growing olive trees in Israel: from abandoned groves to feral and wild?
Raw microsatellite data. The fragment sizes (in base pairs) of the two alleles per individual for each locus are given as a and b (0 represents missing data). (XLSX 38 kb)
MOESM2 of Testing the potential significance of different scion/rootstock genotype combinations on the ecology of old cultivated olive trees in the southeast Mediterranean area
Additional file 2: Table S1. Quantitative phenotypic data for each tree belonging to GG1 and GG2; md represent missing data.