0000000001310206
AUTHOR
R. Gutiérrez
Comportamiento Biológico de Aloinjertos Tendinosos Intraarticulares.
En el presente trabajo, los autores han efectuado un estudio experimental de la respuesta biológica de aloinjertos tendinosos, implantados en el interior de la articulación de la rodilla. Se ha seguido mediante microscopía óptica y micoscopía electrónica, la revascularización de los implantes y los cambios tendinosos intrínsecos, tanto en la serie de aloinjertos en "fresco", como de aloinjertos congelados. En los aloinjertos en fresco, predomina la intensa respuesta inflamatoria -inmunitaria, siendo la respuesta reparadora de escasos signos de orientación del tejido fibroso definitivo. En la serie de injertos congelados, los fenómenos inflamatorios son escasos, predominando la angiogénesis.…
Utilización del Integrated Semen Analysis System (ISAS)® para el análisis morfométrico espermático humano y su significado en las técnicas de reproducción asistida
Use of the integrated semen analysis system (ISAS ® ) for morphometric analysis and its role in assisted reproduction technologies
Facial attractiveness in men provides clues to semen quality
Abstract Facial attractiveness has been related to health in both men and women. Certain psychological, physiological, and secondary sex characteristics have been used as accurate markers of hormonal and developmental health. The main objective of this study was to investigate the capacity of women to select males of high reproductive quality based on their facial attractiveness. A total of 66 males were included in the study. Each of them provides a semen sample, and frontal and lateral photographs were taken. Semen analysis was made according to standard WHO (1999) guidelines for morphology, motility, and concentration. Moreover, a Sperm Index (SI) was calculated as the principal componen…
Regionalization of epididymal duct and epithelium in rats and mice by automatic computer-aided morphometric analysis.
Aim: To establish a rat and mouse epididymal map based on the use of the Epiquatre automatic software for histologic image analysis. Methods: Epididymides from five adult rats and five adult mice were fixed in alcoholic Bouin’s fixative and embedded in paraffin. Serial longitudinal sections through the medial aspect of the organ were cut at 10 µm and stained with hematoxylin and eosin. As determined from major connective tissue septa, nine subdivisions of the rat epididymis and seven for the mouse were determined, consisting of five sub-regions in the caput (rat and mouse), one (mouse) or three (rat) in the corpus and one in the cauda (rat and mouse). Using the Epiquatre software, several t…
Effect of solid storage at 15 degrees C on the subsequent motility and fertility of rabbit semen.
We conducted two studies to improve preservation of rabbit semen. The objective of the first study was determine whether a glucose- and fructose-based extender with two different amounts of gelatin would solidify at 15 degrees C, and to evaluate the influence of gelatin supplementation on sperm motility parameters after storing semen up to 10 days at 15 degrees C. The fertility of rabbit semen diluted in the best gelatin-supplemented extender established in Study 1 and stored for up to 5 days was evaluated in the second study. In Study 1, semen was collected with an artificial vagina from 40 bucks. Each ejaculate was diluted to (80-100) x 10(6) spermatozoa/mL (1:3, semen/extender) at 37 deg…
Comportamiento Biológico de Implantes Tendinosos Autógenos Intraarticulares. Interfase con los distintos medios receptores
En el presente trabajo se ha efectuado un estudio experimental de la respuesta biológica de implantes intraarticulares de tendones autógenos insertados en canales óseos. Se ha seguido, mediante microscopía óptica y electrónica, la revascularización de los implantes, los cambios tendinosos intrínsecos y, fundamentalmente, la interfase establecida entre la estructura tendinosa y los distintos medios del lecho receptor. En las zonas bañadas por líquido sinovial se produce "sinovialización", con presencia de sinoviocitos A (aspecto macrofágico) y B (aspecto fibroblástico). En las zonas que delimitan con cartílago aparece una hendidura, cuya superficie tendinosa es también sinovializada. En la i…
Male Facial Anthropometry and Attractiveness
The symmetry and masculinity of the face are often considered important elements of male facial attractiveness. However, facial preferences are rarely studied on natural faces. We studied the effect of these traits and facial metric parameters on facial attractiveness in Spanish and Colombian raters. In total, 13 metric and 11 asymmetry parameters from natural, unmanipulated frontal face photographs of 50 Spanish men were measured with the USIA semiautomatic anthropometric software. All raters (women and men) were asked to rank these images as potential long-term partners for females. In both sexes, facial attractiveness was negatively associated with facial masculinity, and preference was …
Comparison of three different staining methods for the assessment of epididymal red deer sperm morphometry by computerized analysis with ISAS®
When collection of ejaculated sperm samples is not possible, as is the case with wild species, the epididymides of sacrificed wild males become the only possible source of spermatozoa. Mature cauda epididymal spermatozoa display characteristics similar to those of ejaculated sperm cells. The present work proposes a sperm staining technique suitable for the morphometric evaluation of red deer epididymal sperm using a new computerized system. Epididymides from wild animals were extracted no later than 2 h post mortem. After epididymal sectioning, sperm samples were collected, cooled to and equilibrated at 5 °C, and frozen in liquid nitrogen. Before staining, sperm samples were thawed for 20 s…
Male facial attractiveness and masculinity may provide sex- and culture-independent cues to semen quality
Phenotype-linked fertility hypothesis (PLFH) predicts that male secondary sexual traits reveal honest information about male fertilization ability. However, PLFH has rarely been studied in humans. The aim of the present study was to test PLFH in humans and to investigate whether potential ability to select fertile partners is independent of sex or cultural background. We found that on the contrary to the hypothesis, facial masculinity was negatively associated with semen quality. As increased levels of testosterone have been demonstrated to impair sperm production, this finding may indicate a trade-off between investments in secondary sexual signalling (i.e. facial masculinity) and fertilit…
Use of the Sperm-Class Analyser® for objective assessment of human sperm morphology
The Sperm-Class Analyser was validated for assessing morphometric parameters of the head and midpiece of unwashed and washed human ejaculated spermatozoa from volunteers providing a wide range of semen quality. A higher proportion of sperm could be assessed (86% fresh semen and 75% washed sperm) if Hemacolor staining was used rather than DiffQuik (80 and 73%) or Papanicolaou (78 and 68%). Different stains employed different fixatives and the area, length, width and perimeter of the sperm head was significantly larger for washed sperm stained by Hemacolor and DiffQuik. Acrosomal area ranged from 48 to 51% of the sperm head area and this percentage was larger for washed sperm stained with Dif…
CCDC 1433632: Experimental Crystal Structure Determination
Related Article: G. Hernández, S. Bernès, O. Portillo, A. Ruíz, G.E. Moreno, R. Gutiérrez|2015|Acta Crystallogr.,Sect.E:Cryst.Commun.|71|1462|doi:10.1107/S2056989015020368
CCDC 1433631: Experimental Crystal Structure Determination
Related Article: G. Hernández, S. Bernès, O. Portillo, A. Ruíz, G.E. Moreno, R. Gutiérrez|2015|Acta Crystallogr.,Sect.E:Cryst.Commun.|71|1462|doi:10.1107/S2056989015020368
CCDC 1433633: Experimental Crystal Structure Determination
Related Article: G. Hernández, S. Bernès, O. Portillo, A. Ruíz, G.E. Moreno, R. Gutiérrez|2015|Acta Crystallogr.,Sect.E:Cryst.Commun.|71|1462|doi:10.1107/S2056989015020368