0000000001313940
AUTHOR
Jaime Güemes
Gadoria (Antirrhineae, Plantaginaceae): A new genus, endemic from Sierra de Gádor, Almería, Spain
The surprising discovery of a population of a new species of Antirrhineae (Plantaginaceae) has also given rise to the description of a new genus situated in the subtribe Maurandyinae: Gadoria falukei. This is supported by the characteristics of the flower, pollen grains, seeds and capsule, and phylogenetic results based on DNA sequences. The species is endemic from Sierra de Gádor, Almería, Spain. Scanning electron microscopy was used to explore micromorphology of the capsule, indument, and seed and pollen grains ornamentation. Additionally, chromosomal number, preliminary insights on reproductive biology, phylogenetic position within Antirrhineae, ecological data and conservation status of…
Chromosome numbers of severalLamiaceae from Spain
Chromosome numbers of twenty taxa, almost all of them Iberian endemics, of the generaTeucrium, Sideritis, Thymus andSalvia are reported. New chromosome numbers are given for the following taxa:Teucrium homotrichum (2n=78),T. hifacense (2n=26),T. rivasii (2n=26),T. rivas-martinezii (2n=26),Sideritis edetana (2n=30),S. murgetana subsp.littoralis (2n=28), and the hybridS. xviciosoi (2n=27). The first count on Iberian material forSalvia pratensis (2n=18) is reported. Chromosome numbers have been confirmed for the rest of the taxa studied on material from SE Spain. Karyotype analyses and taxonomic remarks are included.
<p><strong><em>Linaria semialata</em> and <em>L. amethystea </em>subsp.<em> aedoi</em>,<em> </em>two new taxa of <em>L.</em> sect. <em>Supinae </em>(<em>Plantaginaceae</em>) from Southeastern Iberian Peninsula</strong></p>
Two new taxa of the genus Linaria are described, illustrated and compared, through a morphometric analysis, to morphologically similar species from L. sect. Supinae: L. amethystea, L. diffusa, L. intricata, and L. orbensis. A Principal Component Analysis and Linear Discriminant Analysis were carried out in order to find out which morphological characters were the most important to discriminate these species. Morphometric analyses revealed that discrimination of species were more related to their particular morphological character combinations than to a private character. Two taxa are described as new to science, by providing a detailed description, illustrations, scanning-electron micrograp…
A new gypsophilous species ofChaenorhinum(Antirrhinaceae) from the south-east of the Iberian Peninsula
A new strict gypsophilous species of Chaenorhinum, Ch. gamezii, is described. It is an annual to perennial species found in extremely arid locations upon Triassic gypsic habitats in the south-eastern Iberian Peninsula. This species has an eglandular heterotrichous indumentum, which is related to Ch. segoviense and Ch. flexuosum. However, Ch. gamezii has a characteristic combination of vegetative and micromorphological features (trichomes and ornamentation of the seeds) that are not present in any other taxon of the genus. Herein, the affinities and differences with related species are studied and discussed. For its differentiation, an original key is presented with comparative figures of in…
Genetic variability in a narrow endemic snapdragon (Antirrhinum subbaeticum, Scrophulariaceae) using RAPD markers
Antirrhinum subbaeticum is an endangered species inhabiting fragmented limestone cliffs. In the last 3 years, a drastic population decline has been observed in three of four known populations and the estimated number of surviving individuals is now close to 400. A RAPD study was conducted to evaluate the levels of genetic variation present in this species to improve conservation guidelines. Thirty-nine polymorphic products identified 66.1% of the samples by unique RAPD multilocus profiles. A cluster analysis grouped the samples into two broad groups corresponding to northern or southern provenances. AMOVA analysis showed that only 17.7% of the genetic diversity was partitioned within popula…
A new species ofFumana (Cistaceae) from Rif, Morocco
A new species ofFumana (Dunal) Spach subgenusFumana, F. fontqueri, is described from the region of Rif, Morocco. Notes on its morphology, ecology, distribution and taxonomic relationships are presented. It differs fromF. procumbens (Dunal) Gren. etGodr. andF. baetica Guemes, by the indument of the stem, the disposition of pedicels, and the size of the flowers.
Can the problem of hybridization in threatened species be evaluated using a fieldwork research? A case study in snapdragons
Abstract Hybridization, natural or artificial, is considered disadvantageous for species biodiversity when it threatens the population integrity of endangered species. Frequently, studies investigating whether hybridization poses a legitimate risk to rare species are based on genetic data obtained in molecular biology laboratories. In this study, we used field research to approach the problem that hybridization could cause for the viability of a population of a rare species and to be able to propose the most appropriate initial conservation strategy. Specifically, using the model genus Antirrhinum, the reproductive barriers between the rare A. pulverulentum and its common congener A. litigi…
Typification of seven names in the genus Antirrhinum (tribe Antirrhineae, Plantaginaceae)
The typification of seven names in the genus Antirrhinum (tribe Antirrhineae, Plantaginaceae): A. charidemi, A. latifolium, A. molle var. mollissimum, A. molle var. marianum, A. sempervirens, A. siculum, and A. valentinum is discussed. The designation of the nomenclatural types is based on the consultation of original material and the literature cited in the respective protologues. The names are lectotypified using specimens from BC, C, MA, and an illustration of Boccone published in 1697. An epitype is also designated for A. latifolium due to ambiguity in the interpretation of the lectotype. The name A. siculum is neotypified from a specimen preserved at VAL, and a second-step lectotypific…
Pollination biology in an endangered rocky mountain toadflax (Linaria cavanillesii)
Knowledge about the reproductive system of species inhabiting rocky habitats is scarce. The reproductive biology (floral biology, experimental pollination, insect visits, inbreeding depression, and seed predation) of the rupicolous Linaria cavanillesii was analyzed under field and experimental conditions. Self-compatibility was revealed by the high fruit set, seed set, and seed mass in the pollination experiment. Furthermore, results disclose that this species does not need insect visitors for seed production since fruit set after autonomous self-pollination was similar to that by hand cross-pollination. Self-offsprings were not affected by a strong degree of inbreeding depression in early …
Lectotypification of the names of two species of Fumana ( Cistaceae )
The names Cistus ericoides Cav. and C. laevis Cav. are typified. These names apply to two species, currently classified within the genus Fumana (Dunal) Spach, but the names have been frequently misapplied and both of thempose typification problems. The type specimen of the first has not been found. Thus an illustration is chosen as type and an epitype designated. The original material of C. laevis comprises a mixture of two different species. One element is selected to clarify the application of this binomial.
A geographical pattern ofAntirrhinum(Scrophulariaceae) speciation since the Pliocene based on plastid and nuclear DNA polymorphisms
Aim To infer phylogenetic relationships among Antirrhinum species and to reconstruct the historical distribution of observed sequence polymorphism through estimates of haplotype clades and lineage divergence. Location Antirrhinum is distributed primarily throughout the western Mediterranean, with 22 of 25 species in the Iberian Peninsula. Methods Plastid (83 trnS-trnG and 83 trnK-matK) and nuclear (87 ITS) sequences were obtained from 96 individuals representing 24 of the 25 Antirrhinum species. Sequences were analysed using maximum parsimony, Bayesian inference and statistical parsimony networking. Molecular clock estimates were obtained for plastid trnK-matK sequences using the penalized …
Phylogeny of snapdragon species (Antirrhinum; Scrophulariaceae) using non-coding cpDNA sequences
Antirrhinum is an Old World genus of up to 25 perennial taxa, mainly located in the western Mediterranean basin. A molecular analysis of 24 taxa of Antirrhinum was undertaken using cpDNA sequences from the trnT (UGU)-trnL (UAA) 5' exon region. The Kimura two-parameter model was chosen to calculate pairwise nucleotide divergence values between cpDNA sequences, and a bootstrapped neighbor-joining dendrogram was constructed from the nucleotide divergence distance matrix. Eighteen sites were variable across the studied samples and the position of 7 indels, ranging from 1 to 7 bp, was inferred from the sequence alignment. Several trnT-trnL sequences are identical in: some members of subsection K…
The effectiveness of pre- and post-zygotic barriers in avoiding hybridization between two snapdragons (Antirrhinum L.: Plantaginaceae)
Reproductive barriers play an important role in the maintenance of species boundaries. However, to date, few studies have provided a detailed analysis of reproductive isolation barriers between species or examined their importance in maintaining species identity. This is the first detailed study into pre- and post-zygotic reproductive isolation barriers in Antirrhinum, based on a mixed population with two species that rarely co-occur. The study revealed that pollinator constancy and preference and poor hybrid seed viability were the most important reproductive isolating mechanisms. Reproductive isolation was practically complete by both pre- and post-zygotic barriers. Average pre-zygotic is…
Phylogeny, biogeography, and morphological ancestral character reconstruction in the Mediterranean genus Fumana (Cistaceae)
Fumana is a diverse genus of the Cistaceae family, consisting of 21 currently accepted species. In this study, nuclear (ITS) and plastid (matK, trnT‐L) molecular markers were used to reconstruct the phylogeny and to estimate divergence times, including 19 species of Fumana. Phylogenetic analyses (Bayesian Inference, Maximum Parsimony and Maximum Likelihood) confirmed the monophyly of Fumana and did not support the infrageneric divisions previously established. The results support four main clades that group species that differ in vegetative and reproductive characters. Given the impossibility to define morphological characters common to all species within the clades, our proposal is to reje…
The breeding system of Fumana ericifolia: first evidence of autogamy in woody Cistaceae
Fumana ericifolia belongs to the family Cistaceae, in which all perennial species previously studied have been reported to be self-incompatible and xenogamous. Here we show that F. ericifolia is self-compatible and autogamous. Its flowers last only four to eight hours and produce a small amount of pollen and ovules. Self-pollination depends on changes in the relative position of the stigma and the anthers, triggered by the abscission of the petals; this in turn causes closing of the sepals, which push the anthers onto the stigma. Pollen remains highly viable and germinable, and the stigma keeps its receptivity several hours after the loss of the petals. Hand pollination treatments revealed …
Reproductive biology and conservation implications of three endangered snapdragon species (Antirrhinum, Plantaginaceae)
About 32% of Antirrhinum species are considered to be endangered; however, no field studies have focused on their reproductive biology. In this work, several aspects of the reproductive biology (flowering phenology, floral biology, breeding system) and potential limits on seed quantity and quality (pollen limitation, inbreeding depression) were studied in natural populations of three endangered species of the genus (Antirrhinum charidemi, Antirrhinum subbaeticum, Antirrhinum valentinum). Results disclose that all three species need insect visitors for seed production since fruit set after autonomous self-pollination was lower than under hand cross-pollination. A. charidemi and A. valentinum…
Breeding system and conservation strategy of the extremely endangered Cistus carthaginensis Pau (Cistaceae) of Spain
Cistus carthaginensis is one of the most endangered plants in Europe: at present there exist only a few individuals in Murcia and one in Valencia (SE and E Spain). To design an adequate conservation strategy able to avoid the extinction of this species, various aspects of its reproductive biology were studied. The extreme rarity of C. carthaginensis is not related to problems of development and/or fertility of pollen or ovules produced by the few existing specimens. Meiosis in the pollen mother cells is always regular and chromosome segregation is completely equilibrated in the male gametes. Pollen and ovule production is high and similar to that reported for other related species of Cistus…
Nectar robbing does not affect female reproductive success of an endangered Antirrhinum species, Plantaginaceae
ABSTRACTBackground: Nectar robbers in many plant species affect female reproductive success, usually decreasing the amount of seed produced by plants, which can affect populations persistence.Aims:...
Nectar robbing does not affect female reproductive success of an endangered Antirrhinum species, Plantaginaceae
Background: Nectar robbers in many plant species affect female reproductive success, usually decreasing the amount of seed produced by plants, which can affect populations persistence. Aims: To evaluate the impact of nectar robbers on the viability of the populations, we investigated the effects of nectar robbing on the female reproductive success of a threatened snapdragon species in four populations over 2 years. Methods: We observed insect visitors and their behaviour and quantified the percentage of robbed and non-robbed flowers and the production of fruits and seeds. Results: The results showed the degree of variation in nectar robbing among the populations and between years within the…