Metallothionein in the freshwater gastropod Melanopsis dufouri chronically exposed to cadmium: A methodological approach

Previous studies have demonstrated that the use of differential pulse polarography (DPP) for metallothionein (MT) determination in marine gastropod tissues, particularly the digestive gland, requires taking into account the presence of heat-stable high molecular weight compounds that exhibit polarographic signal. In the present paper, similar compounds were identified in tissues from the freshwater snail Melanopsis dufouri which also interfere with MT determination by DPP and, due to their silver binding capacity, also interfere in the silver assay for MT quantification. Ultrafiltration seems to be effective in removing these high molecular weight compounds from heat-denatured homogenate su…

Metal concentrations and detoxification mechanisms in Solea solea and Solea senegalensis from NW Mediterranean fishing grounds

10 pages, 5 figures, 4 tables

Determination of mercury by cold‐vapour technique in several tissues of treated American red crayfish (Procambarus clarkii)

Abstract Adult intermolt specimens of American red crayfish (Procambarus clarkii) collected from Lake Albufera (Valencia, Spain), were exposed to mercury during 96 h. The Hg‐concentrations used were 50, 100, and 250 μg Hg/1 as Cl2Hg. The content of mercury in muscle, midgut gland, antennal glands and gills was investigated. Determinations of mercury were made by cold‐vapour technique and AAS. The mercury levels in all examinated tissues increased significantly (ANOVA test, p < 0.01) with increasing Hg‐concentration in the water.

Effects of sublethal exposure to lead on levels of energetic compounds in Procambarus clarkii (Girard, 1852).

Lead is neither essential nor beneficial to living organisms; all existing data show that its metabolic effects are adverse. Lead is toxic to all phyla of aquatic biota. Most of the lead discharged into surface water is rapidly incorporated into suspended and bottom sediments. The American red crayfish, Procambarus clarkii, lives in a wide range of environmental conditions that include highly polluted waters. Lead present in take sediments can be available to aquatic animals such as P. clarkii because it is a detritivor and burrow into the sediment. In fact, we found remarkable levels of lead in tissues of P. clarkii caught in Albufera Lake and kept 15 days in clean water (e. g. 223 [mu]g/g…

iTRAQ and DIGE in the ecophysiological characterization of zebra mussel populations (Dreissena polymorpha) invading the Ebro and the Júcar hydrographic basins

Poster presentado en el 9th Iberian and 6th Iberoamerican Congress on Environmental Contamination and Toxicology "The environmental research: essential for sustainability" celebrado en Valencia del 1 al 3 de julio de 2013

Proteomic evaluation of potentiated sulfa treatment on gilthead sea bream (Sparus aurata L.) liver

Potentiated sulfa drugs are a combination of sulfonamide and pyrimidine potentiators. They are currently used against fish bacterial pathogens in Mediterranean marine fish farming. The present work aimed studying the potential hepatotoxicity of a combination of sulfadiazine (SDZ) and trimethoprim (TMP) in gilthead sea bream juveniles after oral administration, at the recommended ratio of 5: 1 (SDZ/TMP), equivalent to a dose of 30 mg kg(-1) fish day(-1), for 10 days at 19 degrees C temperature. Electrophoresis (DIGE) technology coupled with MS was used to identify possible markers of hepatotoxicity of this treatment. The results obtained show significant changes in the expression of 41 prote…

Effects of ibuprofen and carbamazepine on the ion transport system and fatty acid metabolism of temperature conditioned juveniles of Solea senegalensis.

The increasing presence of pharmaceuticals in aquatic environments in the last decades, derived from human and veterinary use, has become an important environmental problem. Previous studies have shown that ibuprofen (IB) and carbamazepine (CBZ) modify physiological and biochemical processes in Senegalese sole (Solea senegalensis) in a temperature-dependent manner. In other vertebrates, there is evidence that both of these pharmaceuticals interfere with the ‘arachidonic acid (AA) cascade’, which is responsible for the biosynthesis of numerous enzymes that are involved in the osmoregulatory process. The present work aims to study the temperature-dependent effects of these two pharmaceuticals…

Effects of cadmium exposure on the ultrastructure of hepatopancreatic cells of Thais haemastoma (gastropoda, prosobranchia)

Abstract Specific histopathological parameters have been proposed as good indicators of heavy-metal pollution. Hence the study of ultrastructural alterations in target organs from sentinel animals after heavy-metal exposure may provide a useful tool in understanding the cellular role in heavy-metal metabolism. The main purpose of this study was the determination of the ultrastructural changes in the different hepatopancreatic-cell types of Thais haemastoma after experimental exposure to cadmium. Specimens of this species from the Gulf of Valencia were exposed to sublethal doses of cadmium during a period of 96 h. The hepatopancreas was excised and prepared in a routine manner for electron m…

Changes in biochemical composition of gills, hepatopancreas and muscle of the red crayfish Procambarus clarkii (Girard) after sublethal exposure to mercury.

Abstract 1. 1. The changes in the biochemical composition of gills, hepatopancreas and muscle after exposure to 0.25 mg Hg/l were studied in Procambarus clarkii . 2. 2. Sublethal exposure to mercury in P. clarkii resulted in significant decreases in protein concentration and caloric concentration in gills over the 96 hr period. Glycogen/lipid and glycogen/protein ratios increased after 48 and 96 hr of mercury exposure. 3. 3. Lipid and caloric concentration in the hepatopancreas were significantly lower in 96 hr mercury exposed group. 4. 4. Glycogen concentration in muscle was depleted as consequence of 96 hr mercury exposure.

Effects of salinity acclimation on the proteome of the gilthead seabream (Sparus aurata) heart

Ring test for whole-sediment toxicity assay with -a- benthic marine diatom

7 páginas, 3 figuras, 3 tablas.

Carbamazepine exposure in the sea anemones Anemonia sulcata and Actinia equina: Metabolite identification and physiological responses

Trabajo presentado en el 11º Congreso Ibérico, 8º Iberoamericano de Contaminación y Toxicología (CICTA 2018) celebrado en la Escuela Técnica Superior de Ingeniería de Montes, Forestal y del Medio Natural, Madrid del 11 al 13 de julio de 2018

Comparison of thiol subproteome of the vent mussel Bathymodiolus azoricus from different Mid-Atlantic Ridge vent sites

Deep-sea hydrothermal mussels Bathymodiolus azoricus live in the mixing zone where hydrothermal fluid mixes with bottom seawater, creating large gradients in the environmental conditions and are one of the most studied hydrothermal species as a model of adaptation to extreme conditions. Thiol proteins, i.e. proteins containing a thiol or sulfhydryl group (SH) play major roles in intracellular stress defense against reactive oxygen species (ROS) and are especially susceptible to oxidation. However, they are not particularly abundant, representing a small percentage of proteins in the total proteome and therefore are difficult to study by proteomic approaches. Activated thiol sepharose (ATS) …

Prediction of Aquatic Toxicity of Benzene Derivatives to Tetrahymena pyriformis According to OECD Principles

Background: Many QSAR studies have been developed to predict acute toxicity over several biomarkers like Pimephales promelas, Daphnia magna and Tetrahymena pyriformis. Regardless of the progress made in this field there are still some gaps to be resolved such as the prediction of aquatic toxicity over the protozoan T. pyriformis still lack a QSAR study focused in accomplish the OECD principles. Methods: Atom-based quadratic indices are used to obtain quantitative structure-activity relationship (QSAR) models for the prediction of aquatic toxicity. Our models agree with the principles required by the OECD for QSAR models to regulatory purposes. The database employed consists of 392 substitut…

Effect of sublethal exposure to mercury in the biochemical composition of hepatopancreas in Procambarus clarkii during the recovery after starvation

Abstract Freshwater crayfish undergo seasonal periods of starvation. The hepatopancreas is the main energetic-storage organ in this animal. An adequate replenishment of energetic reserves after these periods of fasting is critical for the proper growth and reproduction in this species. Mercury compounds enter the biological environment from natural discharges and anthropogenic sources. Mercuric chloride is one of the most toxic metallic salts found in polluted waters. In these studies, we report the effect of mercuric chloride in the biochemical composition of crayfish hepatopancreas during the recovery period after prolonged starvation. Adult intermolt crayfish (P. clarkii) were kept starv…

Effect of virgin low density polyethylene microplastic ingestion on intestinal histopathology and microbiota of gilthead sea bream

© 2021 The Authors.

Effects of cadmium on the biochemical composition of the freshwater crayfishProcambarus clarkii (Girard, 1852)

Lake Albufera of Valencia (Spain) and the surrounding rice field waters are subjected to very heavy loads of sewage and toxic residues. Among these residues include heavy metals which have been deposited from the many urban and waste waters in this area. The American red crayfish, Procambarus clarkii from Albufera Lake has high resistance to heavy metals. The authors have also found that P. clarkii shows a high capacity for cadmium accumulation in experimental conditions and natural conditions since crayfish collected in this area contained considerable amounts of cadmium in several tissues even after 15 days of depuration in clean water. Since the physiological changes that take place when…

Effect of ivermectin on the liver of gilthead sea bream Sparus aurata: a proteomic approach.

23 p., 2 tablas, 4 figuras y bibliografía

Short-term exposure of the European sea bass Dicentrarchus labrax to copper-based antifouling treated nets: copper bioavailability and biomarkers responses.

Abstract We studied if the levels of copper released from antifouling treated nets used in finfish mariculture could affect the immune defense mechanism and/or induce oxidative stress in Dicentrarchus labrax , after short term exposure in laboratory experiments. Dissolved copper concentration released from the treated nets, copper bioavailability and a set of biomarkers responses were measured. Biomarkers included hemoglobin concentration, activities of lysozyme, total complement, respiratory burst, glutathione S-transferase and acetycholinesterase and concentration of thiobarbituric acid reactive substances. Results indicated elevated copper concentration in seawater (184 μg L −1 ) but low…

Evaluation of the effects of titanium dioxide and aluminum oxide nanoparticles through tarsal contact exposure in the model insect Oncopeltus fasciatus

Abstract Despite the increasing presence of metal nanoparticles in the biosphere as a consequence of their widespread use, knowledge about the impact of these nanoparticles on fauna, ecosystems and human health is far from completion. This is especially true for terrestrial invertebrates. Insects are environmentally exposed to nanoparticles by several ways, the ectopic contact being one of the most probable. The model insect Oncopeltus fasciatus, has been used in the present work for testing toxicity of nanoparticles present in a surface. Adverse effects of TiO2 nanoparticles and Al2O3 in nanoparticulated or bulk form on mortality, reproductive and embryonic developmental parameters have be…

Effect of cadmium pre-exposure in cadmium accumulation by brine shrimp Artemia: Involvement of low-molecular-weight cadmium-binding ligands

Abstract Cadmium is a ubiquitous non-essential element that possesses high toxicity and is easily accumulated from the environment by aquatic organisms. It is known that pre-exposure to cadmium increases the resistance of animals to a subsequent exposure. In this work, we report studies of the effect of pre-exposure to cadmium on the accumulation of this metal from seawater by brine shrimp Artemia, as well as the involvement of low-molecular-weight cadmium-binding ligands in this process. During the pre-exposure experiments, animals were kept in seawater containing 20 mg/litre Cd for 24 h and then transferred to clean seawater for 48 h. Afterwards, pre-exposed and control animals were expos…

Cadmium-binding proteins in midgut gland of freshwater crayfishProcambarus clarkii

Metallothioneins, metal binding proteins, were originally isolated and characterized by Margoshes and Vallee. These proteins have a high affinity for various heavy metals, particularly cadmium and mercury and have extensively been studied in mammals. Metal binding proteins have been observed in a variety of marine invertebrates; however, there is very little information available on metal binding proteins in freshwater invertebrates, and particularly in freshwater crustaceans. Cadmium is an ubiquitous non essential element which possesses high toxicity to aquatic organisms. Cadmium binding proteins observed in invertebrates have similar characteristics to mammalian metallothioneins. In 1978…

2-D difference gel electrophoresis approach to assess protein expression profiles in Bathymodiolus azoricus from Mid-Atlantic Ridge hydrothermal vents.

Hydrothermal vent mussels Bathymodiolus azoricus are naturally exposed to toxic chemical species originated directly from vent chimneys. The amount of toxic elements varies significantly among vent sites along the Mid-Atlantic Ridge and B. azoricus must be able to adapt to changes in hydrothermal fluid composition, temperature and pressure. The aim of this work was to study changes in the proteome in the "gill-bacteria complex" of mussels B. azoricus from three hydrothermal vent sites with distinct environmental characteristics using 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE). Results showed that 31 proteins had different expression profiles among vent sites and both cluster…

Cadmium binding proteins induced in exposed freshwater crayfish Procambarus clarkii.

This work describes results on the characterization of cadmium binding proteins (Cd-BPs) obtained from cadmium exposed freshwater crayfish Procambarus clarkii. After acclimation to laboratory conditions, induction of Cd-BPs was achieved by water exposure at a concentration of 100 micrograms Cd/L during 2, 15, and 30 d. In accordance with the method followed by Engel and Brouwer, in each case two midgut glands were minced and homogenized in Tris-HC1 buffer with PMSF to prevent protease activity and DTT to maintain reducing conditions. The homogenate was centrifuged, heat treated, applied to a column of Sephadex G-75, and eluted with the same buffer (pH 8.6). Absorbances of the fractions coll…

Histological and electron microscopical observations on the effects of lead on gills and midgut gland ofProcambarus clarkii

Adult intermolt specimen of Procambarus clarkii (Crustacea, Decapoda) have been treated for 96 hours in aquaria with 100mg/l of lead. The gills and midgut gland were processed and studied in the electron microscope. The gill filament shows important changes in the ultrastructure of the epithelial cells and the cuticle. The hepatopancreatic cells studied (i.e. the vacuolated and dark cells) do not show noticeable structural changes but accumulate lead (electrondense precipitates) mainly in lysosomes.

Cadmium, mercury, and lead effects on gill tissue of freshwater crayfishProcambarus clarkii (girard)

Intermolt adult crayfish P. clarkii were used for this work. After acclimatization to laboratory conditions crayfish were exposed to sublethal concentrations of cadmium, mercury, and lead for 96 h. Gills of control and exposed crayfish were removed and ATPase activity and oxygen uptake rate were determined. Structural damage of gill filaments was also observed. Gill tissue respiration rates were measured for individual crayfish using a Gilson differential respirometer. Lead causes a decrease of gill oxygen uptake, but neither cadmium nor mercury seems to affect it at the concentrations employed. Although all metals studied alter gill filament structure, lead damage is the most apparent. In …

Gill ATPase activity in Procambarus clarkii as an indicator of heavy metal pollution.

Lake Albufera and the surrounding rice field waters are subjected to very heavy loads of sewage and toxic industrial residues, including heavy metals, from the many urban and waste waters of this area. The American red crayfish, Procambarus clarkii have a high resistance to toxic effects of heavy metals. The sublethal effects of heavy metals on gills of fish and aquatic invertebrates have been extensively studied. Some metabolic disturbances and histologic damages have been reported, as well as osmoregulation alterations. However, little work has been done about the effect of heavy metals on Na,K and Mg-ATPases of freshwater invertebrate gills. Na,K-ATPase is the prime mediator of ion trans…

Protein expression profiles in Bathymodiolus azoricus exposed to cadmium

Proteomic changes in the "gill-bacteria complex" of the hydrothermal vent mussel B. azoricus exposed to cadmium in pressurized chambers ((Incubateurs Pressurises pour l'Observation en Culture d'Animaux Marins Profonds - IPOCAMP) were analyzed and compared with the non-exposed control group. 2-D Fluorescence Difference Gel Electrophoresis (2D-DIGE) showed that less than 1.5% of the proteome of mussels and symbiotic bacteria were affected by a short-term (24 h) Cd exposure. Twelve proteins of the more abundant differentially expressed proteins of which six were up-regulated and six were down-regulated were excised, digested and identified by mass spectrometry. The identified proteins included…

Oxygen uptake by excised gills ofProcambarus clarkii (Girard) from albufera lake of Valencia, Spain, under heavy metal treatments

The American red crayfish Procambarus clarkii, originally from Louisiana (USA) was introduced in Spain in the 70's in the Guadalquivir River swamps. It appeared first randomly and in a more regular basis afterwards since 1978 in the Albufera Lake south of Valencia and in the surrounding rice fields. Albufera lake and surrounding rice fields waters are being subject since the last three decades to very heavy load of sewage, toxic industrial residues including heavy metals and pesticides from the many urban and industrial settlements in the zone. In the present study, the authors have investigated the effect that heavy metals (Chromium, Cadmium and Mercury) have on the oxygen uptake by excise…

Developmental and Reproductive Effects of Low Cadmium Concentration onArtemiaparthenogenetica

Hatching, reproductive, and lifespan characteristics of an Artemia parthenogenetica population from La Mata (Alicante, Spain) exposed to cadmium were studied. No effects on percentage of cyst hatched nor time of hatching were observed on cysts exposed from 0.01 to 5 mg Cd/L. Cohorts of Artemia were chronically exposed to cadmium in life-table experiments to test whether ecologically relevant impacts may occur after several generations of exposure to a very low concentration of cadmium. Chronic toxicity tests were performed using neonates of a third generation of shrimps exposed to 0.08 mg Cd/L (1/1000 of the LOEC for 24 h acute toxicity test). No significant reduction in the intrinsic rate …

Gold-nanoparticles ingestion disrupts reproduction and development in the German cockroach.

The present work shows the effects of gold nanoparticles (AuNPs) orally administered on reproduction and development of the insect Blattella germanica. Newly emerged females were provided with food containing AuNPs (87.44μg/g) of a size between 15 and 30nm (mean 21.8nm), and were allowed to mate with males. Food ingestion, mortality, reproductive parameters (time to ootheca formation and eclosion, ootheca viability and fertility) as well as postembryonic developmental parameters of the first ootheca (nymphal survival and life span) were recorded throughout the experiment. Gold from AuNPs was accumulated by adults of B. germanica with a bioaccumulation factor of 0.1. Ingestion of AuNPs did n…

Multibiomarker approach to fipronil exposure in the fish Dicentrarchus labrax under two temperature regimes

12 pages, 5 figures, 3 tables, supplementary data https://doi.org/10.1016/j.aquatox.2019.105378

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