0000000001316291
AUTHOR
Ineta Popēna
showing 8 related works from this author
Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes and macrophages.
2018
Abstract. Background Macrophages are one of the most important players in the tumor microenvironment. The polarization status of tumor associated macrophages into a pro-inflammatory type M1 or anti-inflammatory type M2 may influence cancer progression and patient survival. Extracellular vesicles (EVs) are membrane-bound vesicles containing different biomolecules that are involved in cell to cell signal transfer. Accumulating evidence suggests that cancer-derived EVs are taken up by macrophages and modulate their phenotype and cytokine profile. However, the interactions of cancer-derived EVs with monocytes and macrophages at various differentiation and polarization states are poorly understo…
Additional file 5: of Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes a…
2018
Effect of SW480 and SW620-derived EVs on biomolecule secretion patterns of monocytes and M0, M1 and M2 macrophages. Luminex data analysis showing TNFα, IL-6, CXCL10, IL-23, IL-10, MMP9, IL-1β and CCL22 concentration in cell culture supernatants of monocytes (M) and M0, M1 and M2 macrophages following incubation with SW480 and SW620 EVs or without them (control). The graphs represent mean ± SD (n = 3). (PDF 39 kb)
Tumorigenitātes marķieru noteikšana pieaugušo cilmes šūnās in vitro
2013
K-67 un telomerāzes reversā transkriptāze TERT ir olbaltumvielu dabas savienojumi, kuri ir iesaistīti ne tikai šūnu proliferācijas un novecošanas procesos, bet arī onkoģenēzē. Līdzšinējie pētījumi ļauj domāt, ka tos varētu izmantot kā marķierus šūnu tumorigenitātes prognozēšanai. Šī darba mērķis bija noskaidrot, vai un cik lielā mērā šo marķieru aktivitāte ir vērojama pieaugušu cilvēku dermas un kaulu smadzeņu mezenhimālajās cilmes šūnās, kā arī izpētīt iespējamās sakarības starp Ki-67 un TERT aktivitātes līmeni vai starp šo marķieru ekspresijas līmeni un citiem šūnas raksturojošiem parametriem. Darbā izmantota imūnfluorescences metode. Iegūtie rezultāti liecina, ka Ki-67 apgriezti korelē a…
Additional file 3: of Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes a…
2018
Effect of temperature on the SW480 EV uptake in THP-1 monocytes. Flow cytometry histograms showing Syto RNA Select fluorescence intensities of untreated (left) and Syto RNA Select-labeled SW480 EV-treated THP-1 monocytes following incubation at 4 °C (middle) and 37 °C (right). Histogram markers show the percentage of Syto RNA Select-positive cells. (PDF 53 kb)
Betulīnskābes ietekme uz teratokarcinomas šūnu līniju PA-1, plaušu vēža šūnu līniju A549 un adenokarcinomas šūnu līniju HeLa
2011
Betulīnskābe ir triterpēnu grupas savienojums, kuru iespējams izolēt no bērza tāss un kuram piemīt pretaudzēju, anti-HIV un pretvīrusu iedarbība. Šī darba mērķis bija noskaidrot betulīnskābes ietekmi uz teratokarcinomas šūnu līniju PA-1, plaušu vēža šūnu līniju A549 un adenokarcinomas šūnu līniju HeLa. Darbā tika izmantota šūnu iekrāsošana ar propīdija jodīdu un iekrāsoto šūnu analizēšana FACS plūsmas citometrā. Iegūtie dati ļāva precīzi noteikt, kurā šūnas cikla fāzē šūnas atradās, savukārt pēc tā bija iespējams novērtēt, vai pētītajai vielai piemīt citotoksiska ietekme uz noteikto šūnu līniju. Pētījuma rezultāti liecina, ka betulīnskābei piemīt citotoksiska iedarbība uz PA-1 šūnu līniju u…
Additional file 2: of Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes a…
2018
SW480 and SW620-derived EV effect on monocyte (M) and macrophage (M0, M1, M2) viability. a OD values at 450 nm which are in direct proportion of viable cell counts. b SW480 and SW620 EV cytotoxicity on THP-1 monocytes and M0, M1 and M2 macrophages. The graphs represent mean ± SEM (n = 3). Statistical analysis carried out with the t-test. *p ≤ 0.05, **p ≤ 0.01 vs. untreated cell control of the respective monocyte-macrophage cell subset. (PDF 50 kb)
Additional file 4: of Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes a…
2018
TNFα, IL-23, IL-6, IL-1 β, CXCL10, CCL22, IL-10 and MMP9 secretion profile at different monocyte-macrophage differentiation stages. The graphs represent average biomolecule concentrations SEM (n = 3). Statistical analysis carried out with one-way ANOVA test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and **** ≤ 0.0001 vs. untreated cell control of the respective monocyte-macrophage cell subset. (PDF 63 kb)
Additional file 1: of Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes a…
2018
Experimental design of the THP-1 monocyte to macrophage differentiation showing the time points for the addition of EVs and stimulatory molecules. Below the experimental design, representative light microscopy images show morphology of THP-1 monocytes (M), M0 macrophages (M0), M1 macrophages (M1) and M2 macrophages (M2) (n = 4). Scale bar 100 μm. Representative flow cytometry dot plots show CD14, HLA-DR, CD206 and CD68 marker expression at M, M0, M1 and M2 stages. (JPG 1845 kb)