0000000001319677

AUTHOR

Vincenzo Di Marco Lo Presti

showing 9 related works from this author

A High Sensitive Nested PCR for Toxoplasma gondii Detection in Animal and Food Samples

2013

Toxoplasma gondii is a major food and waterborne transmitted parasite world-wide. The tissues and meat samples of many warm blooded animals can contain tissues cysts from chronic toxoplasmosis. Water and vegetable can be contaminated by the parasitic oocysts shed through the feces of infected cats, representing the definitive host of the parasite. A sensitive PCR for Toxoplasma gondii detection is described. The first step amplified the region between the 28S and 18S rDNA in the closely related T. gondii and Neospora caninum; RFLP analysis distinguished the DNA from the two morphologically identical parasites. Although N. caninum is not involved in human transmission, so far, it is importan…

biologyDilution assayfungiNeospora caninumToxoplasma gondiiToxoplasma gondiibiology.organism_classificationmedicine.diseaseApplied Microbiology and BiotechnologyBiochemistryMicrobiologyVirologyNeospora caninumToxoplasmosisparasitic diseasesmedicineParasite hostingRestriction fragment length polymorphismNested polymerase chain reactionFecesRestriction fragments length polymorphismNested PCRBiotechnologyJournal of Microbial & Biochemical Technology
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Biofilm capability of staphylococcus strains isolated from food and the anti-biofilm activity of a chemically synthesized pyrrolomycin

2018


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food isolatespyrrolomycinSettore BIO/19 - Microbiologia GeneraleSettore CHIM/08 - Chimica FarmaceuticaAntibiofilm
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Antibiotic Resistance Profiling, Analysis of Virulence Aspects and Molecular Genotyping of Staphylococcus aureus Isolated in Sicily, Italy

2018

Abstract Staphylococcus aureus is the major cause of foodborne diseases worldwide. In this retrospective study, 84 S. aureus strains were characterized. The collection comprises 78 strains isolated during 1998 and 2014 from dairy products and tissue samples from livestock bred for dairy production in Sicily. One isolate was obtained from a pet (dog), one from an exotic animal (a circus elephant), and four human isolates were obtained during a severe food poisoning outbreak that occurred in Sicily in 2015. All the strains were characterized by pulsed-field gel electrophoresis (PFGE), for antibiotic resistance and presence of toxin genes. PFGE results showed 10 different pulsotypes, with thre…

0301 basic medicineStaphylococcus aureusLivestockantibiotic resistanceGenotypeMLST; MRSA; PFGE; Staphylococcus aureus; antibiotic resistance; toxin genesTetracycline030106 microbiologyVirulenceMRSABiologymedicine.disease_causeSettore BIO/19 - Microbiologia GeneraleApplied Microbiology and BiotechnologyMicrobiologyMicrobiologyFoodborne DiseasesEnterotoxins03 medical and health sciencesAntibiotic resistanceDrug Resistance BacterialPulsed-field gel electrophoresismedicineAnimalsHumansSicilyRetrospective StudiesVirulenceOutbreakOriginal ArticlesPFGEStaphylococcal InfectionsAnti-Bacterial AgentsBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldPenicillin030104 developmental biologyStaphylococcus aureustoxin genesStaphylococcus aureuFood MicrobiologyMultilocus sequence typingAnimal Science and ZoologyMultilocus Sequence TypingMLSTFood Sciencemedicine.drug
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Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

2020

The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five Listeria monocytogenes isolates from a variety of foods and five Escherichia coli isolates from a farm. The E. coli isolates were positive for extended spectrum &beta

esblSettore CHIM/10 - Chimica Degli Alimenti<i>escherichia coli</i>Pharmaceutical ScienceSettore BIO/19 - Microbiologia Generalemedicine.disease_causebiofilmAnalytical ChemistryPersistence (computer science)lcsh:QD241-44103 medical and health scienceschemistry.chemical_compoundMinimum inhibitory concentrationlcsh:Organic chemistryListeria monocytogenesthymolDrug DiscoveryEscherichia colimedicineFood sciencePhysical and Theoretical ChemistryEscherichia coliThymolListeria monocytogene030304 developmental biology0303 health sciences030306 microbiologyOrganic ChemistryBiofilmContaminationchemistryChemistry (miscellaneous)<i>listeria monocytogenes</i>LactamMolecular MedicineMolecules
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Two novel amino acid substitutions in highly conserved regions of prion protein (PrP) and a high frequency of a scrapie protective variant in native …

2019

Background Polymorphisms of the prion protein gene may influence scrapie susceptibility in small ruminants through modified protein conformation. At least 47 amino acid substitutions and 19 silent polymorphisms have been described in goat PRNP reported from several countries. The objective of this study was to investigate PRNP polymorphisms of native Ethiopian goat breeds and compare the results with other goat breeds. Results The analysis of the prion protein gene PRNP in 229 goats belonging to three of the main Ethiopian native goat breeds showed a remarkably high frequency (> 34.6%) of p.(Asn146Ser) in these breeds, a variant involved in scrapie resistance in Cyprus. In addition, two nov…

Genotype040301 veterinary sciencesanimal diseasesScrapieBiologyPrion ProteinPrion ProteinsPRNP0403 veterinary science03 medical and health sciencesSettore AGR/17 - Zootecnica Generale E Miglioramento GeneticoProtein structureEthiopian native goats; novel polymorphismsGoat DiseaseAnimalsGenetic Predisposition to DiseasePrion proteinGene030304 developmental biologyGeneticschemistry.chemical_classification0303 health sciencesGenetic diversityPrPlcsh:Veterinary medicineGoat DiseasesPolymorphism GeneticGeneral VeterinaryAnimalGoatsEthiopian native goatnovel polymorphism04 agricultural and veterinary sciencesGeneral Medicinenervous system diseasesAmino acidchemistryAmino Acid SubstitutionGoatlcsh:SF600-1100PRNPEthiopiaPrion ProteinsResearch ArticleScrapie
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Human leptospirosis cases in Palermo Italy. The role of rodents and climate

2018

Many regions of the world are increasingly exposed to leptospirosis due to poverty, global warming and high urban density. Here, we report a molecular survey for pathogenic Leptospira spp. in rodents and two symptomatic human cases of leptospirosis in the city of Palermo, Italy.Four rodent species were captured in six areas of the city, and a molecular analysis for pathogenic Leptospira spp. on DNA from the kidney samples showed a different prevalence of leptospirosis in all the species of rodents. In addition, two human cases that occurred in May and October of 2009 in the city were also reported. A 67-year-old woman recovered after antibiotic treatment, whereas a 71-year-old woman did not…

DNA Bacterial0301 basic medicinemedicine.medical_specialtyClimateClimate Change030106 microbiology030231 tropical medicinePopulationRodentialcsh:Infectious and parasitic diseases03 medical and health sciences0302 clinical medicineLeptospiraEnvironmental protectionZoonosesEnvironmental healthPrevalencemedicineAnimalsHumansLeptospirosislcsh:RC109-216CitieseducationAgedDisease ReservoirsLeptospiraeducation.field_of_studyHigh prevalencebiologyIncidencelcsh:Public aspects of medicinePublic healthIncidence (epidemiology)ZoonosisPublic Health Environmental and Occupational Healthlcsh:RA1-1270General Medicinebiology.organism_classificationmedicine.diseaseLeptospirosisInfectious DiseasesGeographyItalyFemalePublic HealthCloudburstJournal of Infection and Public Health
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Rickettsia conorii Indian Tick Typhus Strain and R. slovaca in Humans, Sicily

2012

Letter to the Editor.-- et al.

Microbiology (medical)LetterSettore MED/17 - Malattie Infettivevector-borne infectionslcsh:MedicineBacteremiaBiologyTickBoutonneuse FeverMicrobiologylcsh:Infectious and parasitic diseasesBacterial proteinBacterial ProteinsSequence Homology Nucleic AcidmedicineHumanslcsh:RC109-216Letters to the EditorSicilyCiencias VeterinariasStrain (biology)RICKETTSIOSIS SICILY TICKlcsh:RMediterranean spotted feverMediterranean spotted fever (Boutonneuse fever)zoonosisIndian tick typhus strainmedicine.diseasebiology.organism_classificationVirologyrickettsiatickRickettsia slovacaBoutonneuse feverRickettsia conoriiInfectious DiseasesCIENCIAS AGRÍCOLASRickettsia slovacaepidemiology//purl.org/becyt/ford/4.3 [https]Rickettsia conorii//purl.org/becyt/ford/4 [https]TyphusMultilocus Sequence TypingEmerging Infectious Diseases
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Additional file 2: of Two novel amino acid substitutions in highly conserved regions of prion protein (PrP) and a high frequency of a scrapie protect…

2019

Table of the genotypes combinations of Prion protein gene in native Ethiopian goats.. (PDF 423 kb)

body regionsnervous systemfungi
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Additional file 1: of Two novel amino acid substitutions in highly conserved regions of prion protein (PrP) and a high frequency of a scrapie protect…

2019

Prion protein gene polymorphism in native Ethiopian goats. Excel file that include all information related to each single goat in the three Ethiopian regions. For each goat, information regarding sex, age in months and the related prion polymorphisms are reported. (XLS 60 kb)

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