Energiatehokkaan mobiilisovellusohjelmoinnin välineitä

Älypuhelimista on tullut suosittuja ja ne ovat kehittyneet nopeasti, tarjoten jatkuvasti tehokkaampia suorittimia sekä nopeampia langattomia verkkotekniikoita. Laitteiston kehittyminen on luonut markkinat entistä monipuolisemmille ja kehittyneemmille mobiilisovelluksille, mutta mobiililaitteiden käytettävyys riippuu kokonaan niiden akun mahdollistamasta käyttöajasta. Niinpä sovelluskehittäjien on pystyttävä tarjoaman energiatehokkaita sovelluksia, joissa on silti monimutkaisia toimintoja. Energiatehokkaiden sovellusten kehittäminen vaatii kuitenkin energiatehokkaan ohjelmoinnin käytänteitä ja menetelmiä valittujen ratkaisujen energiankulutuksen arviointiin. Tässä työssä suoritetaan kattava …

Photochemical mineralization of terrigenous DOC to dissolved inorganic carbon in ocean

When terrigenous dissolved organic carbon (tDOC) rich in chromophoric dissolved organic matter (tCDOM) enters the ocean, solar radiation mineralizes it partially into dissolved inorganic carbon (DIC). This study addresses the amount and the rates of DIC photoproduction from tDOC and the area of ocean required to photomineralize tDOC. We collected water samples from 10 major rivers, mixed them with artificial seawater, and irradiated them with simulated solar radiation to measure DIC photoproduction and the photobleaching of tCDOM. The linear relationship between DIC photoproduction and tCDOM photobleaching was used to estimate the amount of photoproduced DIC from the tCDOM fluxes of the stu…

Photochemical Mineralization of Terrigenous DOC to Dissolved Inorganic Carbon in Ocean

DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen

Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a si…

Comparing the Ecotoxicological Effects of Perfluorooctanoic Acid (PFOA) and Perfluorohexanoic Acid (PFHxA) on Freshwater Microbial Community

The ubiquitous presence of perfluorinated carboxylic acids (PFCAs) around the globe has attracted increasing attention, due to their persistency, bioaccumulation, and toxicity. Nevertheless, the ecotoxicological effects of the compounds on aquatic microorganisms has remained understudied. Hence, the present study focused on determining, and comparing, the effects of regulated long-chain PFCA, perfluorooctanoic acid (PFOA), and nonregulated short-chain PFCA, perfluorohexanoic acid (PFHxA), on the diversity, structure, microbial growth, and activity of a freshwater microbial community. In the experiment, lake water was incubated for a period of four weeks at three different concentrations of …

Supplementary figure: DNA recovery from Droplet Digital PCR emulsions using Liquid Nitrogen

Supplementary figure 1 – Applicability of LN2 method to break ddPCR emulsion. ddPCR reactions were prepared with ddPCR™ Supermix for Probes (no dUTP) and Droplet Generation Oil for Probes (three tubes on the left) or ddPCR™ Supermix for EvaGreen® and Droplet Generation Oil for EvaGreen® (three tubes on the right). The tubes (A) and (D) show the emulsion before using LN2 method, and tubes (B), (C), (E) and (F) shows the oil and water layers after breaking the emulsion.

Supplementary table: DNA recovery from Droplet Digital PCR emulsions using Liquid Nitrogen

Supplementary table - Raw data from experiments studying DNA recovery from droplets using chemical and physical procedures.

Original data for article: Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton

The dataset is divided into four subfolders: 1) "SEM experiment data" contains Scanning Electron Microscopy data, epifluorescence microscopy data and flow cytometry data of cultured Synechococcus, Chroococcus and Snowella 2) "raw data" contains epifluorescence microscopy and flow cytometry data of picophytoplankton from Finnish lakes. This has two sub folders "flow cytometry raw" and "microscopy raw" 3) "flow cytometry calibration data" contains data for cell size calibration with latex beads and volumetric calibration for the flow cytometer 4) "processed flow and microscopy data" contains excel workbooks for the figures shown in the manuscipt