6533b7cefe1ef96bd125717e

RESEARCH PRODUCT

Cytoprotective effect of resveratrol diastereomers in CHO-K1 cells exposed to beauvericin

Vincenzo BrandoliniBeatriz MallebreraGuillermina FontMaría-josé Ruiz

subject

Antioxidantvirusesmedicine.medical_treatmentCHO CellsResveratrolToxicologymedicine.disease_causeLipid peroxidationchemistry.chemical_compoundCricetulusCricetinaeDepsipeptidesStilbenesmedicineAnimalsViability assayCytotoxicitychemistry.chemical_classificationReactive oxygen speciesChemistryvirus diseasesGeneral Medicinerespiratory systemMolecular biologyBeauvericinBiochemistryCytoprotectionResveratrolLipid PeroxidationReactive Oxygen SpeciesOxidative stressFood Science

description

Beauvericin (BEA) causes cytotoxicity, lipid peroxidation and reactive oxygen species in CHO-K1 cells. Resveratrol (RSV) is a polyphenol with multiple biological properties, including antioxidant effects. RSV has two forms: trans and cis. The aims of this study were to determine the cytoprotective effect of trans-RSV and diastereomers mixtures (50:50 trans/cis-RSV and 70:30 trans/cis-RSV) incubated alone and in combination with BEA in ovarian (CHO-K1) cells. The results demonstrated that cell viability increases (from 9% to 77%) when they were exposed to low concentration of RSV. Moreover, when the cells were pre-treated with RSV and then exposed to BEA, a cytoprotective effect (from 25% to 76%) and a ROS production diminution (from 27% to 92%) were observed, with respect to cells exposed to BEA without previous RSV exposure. RSV pre-treatment decreased the MDA levels (from 15% to 37%) when it is compared with cells exposed only to BEA. Therefore, it can be concluded that RSV could reduce the toxicological risk produced by BEA when they are in combination.

yearjournalcountryeditionlanguage
2015-06-01Food and Chemical Toxicology
https://doi.org/10.1016/j.fct.2015.03.028