6533b7cefe1ef96bd1257b55
RESEARCH PRODUCT
Alternative Polyadenylation Events Contribute to the Induction of NF-ATc in Effector T Cells
Michael ZimmerJudith GlöcknerInna InashkinaInna InashkinaCornelia EscherAndreas KerstanEdgar SchmittEdgar SerflingEdgar SerflingFriederike Berberich-siebeltSergei ChuvpiloAndris AvotsEriks JankevicsChristian Fischersubject
Gene isoformPolyadenylationImmunologyMolecular Sequence DataGene inductionBiologyLymphocyte ActivationTransfectionT-Lymphocytes RegulatoryJurkat CellsMiceGenes ReporterCritical thresholdTumor Cells CulturedImmunology and AllergyAnimalsHumansAmino Acid SequenceCloning MolecularLuciferasesTranscription factormRNA Cleavage and Polyadenylation FactorsCleavage stimulation factorBase SequenceNFATC Transcription FactorsEffectorNuclear ProteinsRNA-Binding ProteinsMolecular biologyDNA-Binding ProteinsInfectious DiseasesPoly ATranscription Factorsdescription
Abstract The transcription factor NF-ATc is synthesized in three prominent isoforms. These differ in the length of their C terminal peptides and mode of synthesis. Due to a switch from the use of a 3′ polyA site to a more proximal polyA site, NF-ATc expression switches from the synthesis of the two longer isoforms in naive T cells to that of short isoform A in T effector cells. The relative low binding affinity of cleavage stimulation factor CstF-64 to the proximal polyA site seems to contribute to its neglect in naive T cells. These alternative polyadenylation events ensure the rapid accumulation of high concentrations of NF-ATc necessary to exceed critical threshold levels of NF-ATc for gene induction in effector T cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-02-01 | Immunity |