6533b7cffe1ef96bd125852b
RESEARCH PRODUCT
Molecular characterisation of the species of the genus Zygosaccharomyces.
Braulio Esteve-zarzosoBraulio Esteve-zarzosoAmparo QuerolCarmela BellochTina Zormansubject
GeneticsPhylogenetic treebiologyZygosaccharomycesChromosomeKaryotypeGenes rRNAZygosaccharomycesbiology.organism_classificationApplied Microbiology and BiotechnologyMicrobiologyDNA MitochondrialPolymerase Chain ReactionRestriction fragmentRNA Ribosomal 5.8SGenusKaryotypingMolecular phylogeneticsDNA Ribosomal Spacerbiology.proteinRestriction fragment length polymorphismChromosomes FungalDNA FungalEcology Evolution Behavior and SystematicsPolymorphism Restriction Fragment Lengthdescription
The restriction fragments polymorphisms of the mitochondrial DNA and the PCR fragment that comprised the internal transcribes spacers and the 5.8S rRNA gene, together with the electrophoretic karyotypes of 40 strains from the 10 species of the genus Zygosaccharomyces, including the new species Z. lentus were examined. The RFLP's of the ITS-5.8S region showed a specific restriction pattern for each species, including the new species Z. lentus. The only exception were the species Z. cidri and Z. fermentati that produced identical restriction profiles. The electrophoretic chromosome patterns confirmed the differences between the species of this genus, including the phylogenetic closest species Z. cidri and Z. fermentati. They present few chromosomes ranging from 3 bands (4 or 5 chromosomes) for Z. florentinus to 7 bands (8 to 10 chromosomes) for Z. cidri and Z. fermentati. The strain level resolution power of RFLP's of mtDNA of this genus enabled the characterisation of strains from the same species, even where they are isolated from the same substrate. However, in the cases of Z. bailii and Z. lentus, electrophoretic karyotyping there was considerable variation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-10-08 | Systematic and applied microbiology |