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RESEARCH PRODUCT
Cutting Edge: An IL-17F-CreEYFP Reporter Mouse Allows Fate Mapping of Th17 Cells
Andrew L. CroxfordAri WaismanFlorian C. Kurschussubject
Yellow fluorescent proteinAdoptive cell transferEncephalomyelitis Autoimmune ExperimentalRNA UntranslatedTransgeneImmunologyCre recombinaseMice TransgenicCD8-Positive T-LymphocytesT-Lymphocytes RegulatoryImmunophenotypingMiceBacterial ProteinsGenes ReporterFate mappingAnimalsHumansImmunology and AllergyCytotoxic T cellCells CulturedIntegrasesbiologyInterleukin-17ProteinsCell DifferentiationAdoptive TransferMolecular biologyPhenotypeIn vitroMice Inbred C57BLLuminescent ProteinsGene Expression RegulationMice Inbred DBAbiology.proteindescription
Abstract The need for reporter lines able to faithfully track Th17 cells in vivo has become an issue of exceptional importance. To address this, we generated a mouse strain in which Cre recombinase is expressed from the IL-17F promoter. Crossing the IL-17F-Cre allele to a conditional enhanced yellow fluorescent protein (EYFP) reporter mouse yielded the IL-17F-CreEYFP strain, in which IL-17F expression is twinned with EYFP in live IL-17F-expressing cells. Although we demonstrate that IL-17F expression is restricted to CD4+ T cells during experimental autoimmune encephalomyelitis, IL-17F-CreEYFP CD8 T cells robustly expressed IL-17F in response to TGF-β, IL-6, and IL-23. Fate mapping of IL-17F-expressing reporter T cells revealed a significant down-regulation of Th17 cytokines after homeostatic expansion in RAG1-deficient animals. Despite this loss of effector phenotype, committed Th17 cells were resistant to Foxp3 expression in vitro or in vivo. Thus, the IL-17F-Cre strain furthers our understanding of Th17 biology.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-02-01 | The Journal of Immunology |