6533b7cffe1ef96bd12585ba
RESEARCH PRODUCT
Kinetics of in vivo inhibition of tissue cathepsin d by pepstatin A
Luciano RausaNicola GebbiaGaetano LetoFrancesca Maria Tumminellosubject
Pepstatin Amedicine.medical_treatmentPeriod (gene)KineticsCathepsin DBiochemistryCathepsin DMicechemistry.chemical_compoundIn vivoPepstatinsmedicineAnimalsProteasebiologyMusclesMyocardiumSkeletal muscleEnzyme assayKineticsmedicine.anatomical_structureLiverchemistryBiochemistrybiology.proteinFemaleProteinase InhibitorsOligopeptidesPepstatindescription
1. 1. We have investigated the kinetics of inhibition of cathepsin D in heart, liver and skeletal muscle of CD-1 mice following administration of 25, 50, 100 and 200 mg/kg i.p. of pepstatin A, a specific inhibitor of this protease. 2. 2. In the liver, a significant inhibition of cathepsin D occurred up to at least 15 days, whereas, in heart and skeletal muscle, this inhibition lasted for a much shorter period of time. 3. 3. These results show that the recovery of enzyme activity to normal values is dose-dependent and that, at the same dose level, marked differences occur in the recovery of enzyme activity in these organ tissues, the liver being the most sensitive one. © 1988.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-01-01 |