6533b7cffe1ef96bd1258cef
RESEARCH PRODUCT
In vivoInvestigation of Plant-Cell Metabolism by means of natural-abundance13C-NMR spectroscopy
Ralf LutterbachJoachim Stöckigtsubject
SucroseHydroquinoneMetaboliteOrganic ChemistryArbutinMetabolismPlant cellBiochemistryCatalysisInorganic Chemistrychemistry.chemical_compoundchemistryBiochemistryIn vivoDrug DiscoveryPhysical and Theoretical ChemistrySugardescription
Based on the natural abundance of 13C, in vivo13C-NMR was used for the first time to monitor the metabolism of sucrose and hydroquinone (1) in cell suspensions of the plant Rauwolfia serpentina (L.) BENTH. ex KURZ. Cells converted sucrose extracellularly into α-D- and β -D-glucose as well as into β -D-fructofuranose and β -D-fructopyranose, respectively. The sugar mixture was completely taken up by the cells after 4 days. Hydroquinone fed at that time resulted in optimum conversion into its β -D-glucoside arbutin (2) within 10 h. A further metabolite, the primeveroside (3) of hydroquinone, appeared as a trace compound after 10 h. The formation of this diglycoside can be increased by further addition of sucrose.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-12-14 | Helvetica Chimica Acta |