6533b7cffe1ef96bd1258f0c
RESEARCH PRODUCT
Even free radicals should follow some rules: a guide to free radical research terminology and methodology.
Giovanni E. MannBalaraman KalyanaramanKelvin J.a. DaviesOhara AugustoJose ViñaL. Jackson RobertsRafael RadiPhyllis A. DenneryHarry IschiropoulosHenry Jay FormanHenry Jay FormanRegina Brigelius-flohésubject
Biological studiesFree RadicalsChemistryRadicalRADICAIS LIVRESFree Radical ScavengersBiochemistryReactive Nitrogen SpeciesThiobarbituric Acid Reactive SubstancesPhysiological responsesAntioxidantsTerminologyLipid peroxidationF2-Isoprostanechemistry.chemical_compoundBiochemistryPhysiology (medical)Terminology as TopicAnimalsHumansBiochemical engineeringLipid PeroxidationReactive Oxygen SpeciesFluorescent Dyesdescription
Free radicals and oxidants are now implicated in physiological responses and in several diseases. Given the wide range of expertise of free radical researchers, application of the greater understanding of chemistry has not been uniformly applied to biological studies. We suggest that some widely used methodologies and terminologies hamper progress and need to be addressed. We make the case for abandonment and judicious use of several methods and terms and suggest practical and viable alternatives. These changes are suggested in four areas: use of fluorescent dyes to identify and quantify reactive species, methods for measurement of lipid peroxidation in complex biological systems, claims of antioxidants as radical scavengers, and use of the terms for reactive species.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-06-20 | Free radical biologymedicine |