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RESEARCH PRODUCT

Isolation of seven polymorphic microsatellites in Ophioblennius atlanticus atlanticus (Perciformes, Blenniidae)

Alessandro GrapputoM. De GirolamoRicardo S. SantosAlberto PallaviciniLorenzo Zane

subject

GeneticsEcologybiologyZoologybiology.organism_classificationBiochemistrySSRGeneral Biochemistry Genetics and Molecular BiologyPerciformesLoss of heterozygositySSR; POPULATION GENETICS; blennidaePolymorphic microsatellitesPOPULATION GENETICSOphioblennius atlanticusMicrosatelliteblennidae

description

We isolated and characterized seven polymorphic microsatellite loci of Ophioblennius atlanticus atlanticus (Valenciennes, 1836) using an optimized protocol to construct and screen a microsatellite-enriched genomic library. The analysis of variability was performed in 16 specimens from Faial Island (Azores, Portugal). The mean number of alleles was 8.71 ± ± ± 2.43 and the level of expected heterozygosity ranged from 0.764 to 0.903. The total exclusionary probabilities using these loci for the first and the second parent were 0.985 and 0.998, respectively, suggesting that these microsatellites are a useful tool for large-scale parentage analysis.

yearjournalcountryeditionlanguage
2005-02-28
10.1111/j.1471-8286.2005.00918.xhttp://hdl.handle.net/11577/2439819