6533b7cffe1ef96bd125912d
RESEARCH PRODUCT
Analysis of Chlorpyrifos in Water, Fruit Juice, and Honeybee Extract by Chemiluminescent Elisa
Severino GhiniCarla SolerJordi MañesFabiana FiniAngel MontoyaStefano GirottiJuan J. Manclússubject
Detection limitChromatographyChemistryBiochemistry (medical)Clinical BiochemistryFOOD/FRUIT JUICESBiochemistryCHEMILUMINESCENCECHLORPYRIFOSAnalytical Chemistrylaw.inventionStandard curveHONEYBEEchemistry.chemical_compoundlawChlorpyrifosElectrochemistryELISAFruit juiceIC50SpectroscopyChemiluminescencedescription
Abstract The suitability of competitive enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection-based immobilized antigen (indirect assay) for rapid and accurate determination of chlorpyrifos in various food matrices was tested. The limit of detection (LOD) values were 1–1.75 ng mL−1, the standard curve midpoint (IC50) was 3.5 ng mL−1, and the assay duration was 1.5 h. Assay application to the analysis of honeybee extract resulted in chlorpyrifos recoveries varying between 62 and 83% in 5–15 ng mL−1 herbicide concentration range.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-10-31 | Analytical Letters |