6533b7cffe1ef96bd12597ac

RESEARCH PRODUCT

SENSITIZED LANTHANIDE FLUORESCENCE DETECTION OF STEROIDAL HORMONES

subject

description

Several steroidal hormones with an α,β-unsaturated carbonyl group sensitized the fluorescence of terbium(III) ions: medroxyprogesterone, medroxyprogesterone acetate, methyltestosterone, nandrolone, nandrolone decanoate, progesterone, testosterone, testosterone enanthate, and testosterone propionate, but other steroids which have this chromophore did not give any signal: clostebol acetate, dehydrotestosterone, dydrogesterone, methandienone, and methenolone enanthate. Sensitized fluorescence was used to detect the steroids when eluted with micellar mobile phases of sodium dodecyl sulphate (SDS)-acetonitrile and SDS-pentanol containing 0.01 M Tb(III), with 245 nm and 545 nm as excitation and emission wavelengths, respectively. The micelles favour the fluorescence process due to proximity of donor and acceptor, and shielding of the Tb(III) ions from the aqueous medium. Signal intensity increased linearly with the concentration of Tb(III), but decreased with acetonitrile and pentanol. The concentration of orga...