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RESEARCH PRODUCT
Bis(hydroxyphenyl)methane-bisphenol F-metabolism by the HepG2 human hepatoma cell line and cryopreserved human hepatocytes
Georges De SousaRoger RahmaniCoralie DumontMarie-christine ChagnonLaurent DebrauwerElisabeth PerduJean-pierre Cravedisubject
Bisphenol FHealth Toxicology and MutagenesisestrogenicityCell Culture Techniques010501 environmental sciencesToxicology01 natural sciencesMass SpectrometryCryopreservationchemistry.chemical_compoundenzyme level[SDV.IDA]Life Sciences [q-bio]/Food engineeringperformance liquid chromatographyratLuciferasesinductionChromatography High Pressure Liquidendocrine disruptor0303 health sciencesfood and environmental contaminantMolecular StructureHep G2 CellsGeneral MedicineBiochemistryHepg2 cellsin vitro modeldispositionToxicityEnvironmental Pollutantsliver enzymebiotransformationGlucuronidePlasmidsBiologyTransfectionliver03 medical and health sciencesHumans[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringBenzhydryl Compounds030304 developmental biology0105 earth and related environmental sciencesCryopreservationPharmacologyChemical Health and Safetyactivitybisphenol aEstrogen Receptor alphaPublic Health Environmental and Occupational HealthMetabolismbeta-GalactosidaseHepatoma cell linechemistryHepatocytesXenobioticdescription
author cannot archive publisher's version/PDF; International audience; Bisphenol F (BPF) is present in the environment and as a contaminant of food. Humans may, therefore, be exposed to BPF, and an assessment of this risk is required. BPF has been shown to have genotoxic and endocrine-disruptor properties in a human hepatoma cell line (HepG2), which is a model system for studies of xenobiotic toxicity. In this study, we investigated the ability of HepG2 cells to biotransform BPF, because metabolism may affect the observed effects of BPF, and we compared this metabolic capacity with that of human hepatocytes. Cells were incubated for 24 hours with [(3)H]-BPF. The culture medium was then concentrated and its metabolites were isolated by high-performance liquid chromatography and identified by mass spectrometry. BPF was largely metabolized into the corresponding sulfate by the HepG2 cell line. BPF was metabolized into both sulfate and glucuronide by human hepatocytes, but with differences between individuals. The metabolism of BPF in both HepG2 cells and human hepatocytes suggests the existence of a detoxification pathway. Thus, these two cell models differ in metabolic capacity. It is, therefore, very important, when assessing the toxic effects of substances in vitro, to determine, in parallel, the biotransformation capacities of the model used to extrapolate in vivo.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-01-01 |