6533b7cffe1ef96bd1259980

RESEARCH PRODUCT

Lung Compartmentalization of Increased TNF Releasing Ability by Mononuclear Phagocytes in Pulmonary Sarcoidosis

Giovanni BonsignoreAnna Maria MerendinoMario SpataforaVincenzo BelliaMark GjomarkajG. ChiapparaM. Melis

subject

AdultLung DiseasesMalePulmonary and Respiratory MedicineSarcoidosisCritical Care and Intensive Care MedicinePathogenesisHumansMedicineMacrophageLungLungTumor Necrosis Factor-alphabusiness.industryMacrophagesRespiratory diseaseMononuclear phagocyte systemCytotoxicity Tests Immunologicmedicine.diseasePulmonary AlveoliMonokinemedicine.anatomical_structureImmunologyLeukocytes MononuclearFemaleTumor necrosis factor alphaSarcoidosisCardiology and Cardiovascular MedicinebusinessBronchoalveolar Lavage Fluid

description

The TNF is a monokine with cytotoxic and tumor-necrosing activities; in addition, TNF may play a role in inflammatory processes. The present study evaluates spontaneous and LPS-mediated release of TNF by AMs and autologous peripheral BMs of normal subjects and patients with pulmonary sarcoidosis. A recently developed cytotoxicity assay, specific for detection of TNF activity, was applied. This study demonstrates that (1) unstimulated mononuclear phagocytes released low levels of TNF with no differences between groups; (2) when effector cells were stimulated with LPS, AMs from patients with active pulmonary sarcoidosis released more TNF than AMs recovered from normal subjects and from patients with inactive disease; (3) this increase was compartmentalized to the lungs, since comparisons of TNF production by LPS-stimulated BMs failed to show any difference between study groups. These results suggest that TNF might play a role in the pathogenesis of the alveolitis of pulmonary sarcoidosis.

yearjournalcountryeditionlanguage
1989-09-01Chest
https://doi.org/10.1378/chest.96.3.542