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RESEARCH PRODUCT
Antibacterial effect of the bioactive compound beauvericin produced by Fusarium proliferatum on solid medium of wheat.
Antonio MorettiJordi MañesJose SorianoGiuseppe MecaAlberto RitieniGiuseppe MecaIsabel Sospedrasubject
Spectrometry Mass Electrospray IonizationFusarium proliferatumToxicologymedicine.disease_causeHigh-performance liquid chromatographymycotoxinchemistry.chemical_compoundFusariumTandem Mass SpectrometryLiquid chromatography–mass spectrometryDepsipeptideswheatmedicineTriticumAntibacterial agentChromatographybiologybeauvericinfood and beveragesClostridium perfringensbiology.organism_classificationBeauvericinBioactive compoundAnti-Bacterial AgentschemistrySolid-state fermentationSpectrophotometry UltravioletChromatography Liquiddescription
To obtain the bioactive compound beauvericin (BEA), Fusarium proliferatum CECT 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (SSF), being this mycotoxin purified by high performance liquid chromatography (HPLC) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. The purity of the BEA was verified by analytical HPLC and liquid chromatography tandem mass spectrometry (LC/MS-MS). The pure fractions of BEA were utilized to determinate the antibiotic effects on several bacterial strains that are considered normally pathogens of the intestinal tract as: Escherichia coli, Enterococcus faecium, Salmonella enterica, Shigella dysenteriae, Listeria monocytogenes, Yersinia enterocolitica, Clostridium perfringens, Pseudomonas aeruginosa and Staphylococcus aureus.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-01-01 |