6533b7d0fe1ef96bd125a1bc

RESEARCH PRODUCT

Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes via Proximity Ligation Assay

Katharina WolfSusanne Strand

subject

0301 basic medicine03 medical and health sciences030104 developmental biologyIn vivoChemistryCell cultureProximity ligation assayHistone deacetylase activityHistone deacetylaseTransfectionMolecular biologyGene knockoutIn vitro

description

Generation of primary cell culture of hepatocytes by mouse liver perfusion (MLP) combines the advantages of in vivo and in vitro models. It provides hepatocytes that grow under physiological conditions in mice, with the genotype of the whole organism or a specific gene knockout. In contrast to immortalized cell cultures, primary murine hepatocytes (pmHep) are non-cancerous cells with a limited lifespan but still amenable to classical in vitro methods such as treatment with drugs, small molecule inhibitors, and agonistic/antagonistic antibodies of surface receptors as well as transfection. One technique, which has gained popularity recently, is the analysis of protein-protein interactions by the proximity ligation assay (PLA). Here, we describe a liver perfusion protocol and the detection of the histone deacetylase function of Sirtuin 6 (SIRT6) using PLA in pmHep.

yearjournalcountryeditionlanguage
2016-10-20
https://doi.org/10.1007/978-1-4939-6527-4_11