6533b7d0fe1ef96bd125a225

RESEARCH PRODUCT

The cAMP Pathway Sensitizes VR1 Expressed in Oocytes from <i>Xenopus laevis</i> and in CHO Cells

Clemens GillenCaroline WiwieSebastian D. VulcuRuth JostockJohanna RuppHermann Nawrath

subject

Pharmacologymedicine.medical_specialtyForskolinbiologyChinese hamster ovary cellTRPV1XenopusGeneral Medicinebiology.organism_classificationCalcium in biologyCell biologychemistry.chemical_compoundEndocrinologychemistryCapsaicinInternal medicinemedicinecAMP-dependent pathwaylipids (amino acids peptides and proteins)Protein kinase C

description

The vanilloid receptor 1 (VR1) is a heat-activated cation channel which also responds to capsaicin and other chemical stimuli. Protein kinase C has a stimulatory effect on VR1 activity, either alone or after activation with capsaicin. The influence of the cAMP-signaling pathway on the effects of capsaicin is controversial. To clarify this, the actions of capsaicin and the modulatory effects of forskolin, pCPT-cAMP, and isobutylmethylxanthine were studied in <i>Xenopus laevis</i> oocytes expressing rat VR1 and in CHO cells expressing human VR1. Capsaicin activated the VR1 channel and increased the intracellular calcium concentration. The effects of capsaicin were enhanced by forskolin, pCPT-cAMP, and isobutylmethylxanthine. A modulatory function of the cAMP system on VR1 activation could, therefore, modulate heat sensation and pain.

yearjournalcountryeditionlanguage
2003-01-01Pharmacology
https://doi.org/10.1159/000071265