6533b7d0fe1ef96bd125a464
RESEARCH PRODUCT
Oxidation of adrenaline and noradrenaline by solved molecular oxygen in a FIA assembly.
A. Mellado RomeroJ. Martínez CalatayudS.garcı́a Palopsubject
Detection limitReaction mechanismChromatographyEpinephrineChemistryClinical BiochemistryAnalytical chemistryFluorescence spectrometryPharmaceutical ScienceFluorescence spectroscopyAnalytical ChemistryOxygenNorepinephrineLinear rangeDrug DiscoveryFlow Injection AnalysisFluorometryMolecular oxygenQuantitative analysis (chemistry)Oxidation-ReductionSpectroscopyAnalysis methoddescription
A simple and effective procedure is proposed for the study and simultaneous determination of adrenaline and noradrenaline. The fluorimetric determination of both substances is performed in a flow injection assembly and by oxidation of both drugs with the solved molecular oxygen. The influence of different parameters is empirically studied and the interpretation of the reaction mechanism is also added. The determination of adrenaline is monitored at 450 nm and the outputs at 520 nm correspond to the adrenaline and noradrenaline global amount; for both lectures lambda(exc) 329 nm. The influence of temperature is relevant and analytical determination occurred at 55 degrees C by immersing the sample loop in a water bath. The linear range for adrenaline is over 0.5-20 microg ml(-1), limit of detection for both compounds is 0.2 microg ml(-1): the influence of foreign compounds as potential interferents is also tested; and, finally the procedure is applied to determination of both chatecolamines in synthetic samples.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-03-01 | Journal of pharmaceutical and biomedical analysis |