6533b7d0fe1ef96bd125ab75

RESEARCH PRODUCT

Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins

Hervé LucheHans Joerg FehlingSebastian AttigAndré P. HeinenFlorian C. KurschusSonja MoosPrajna Paramita PalNediljko BudisaAri WaismanFlorian Wanke

subject

animal structuresHistologymedicine.diagnostic_testmedicine.medical_treatmentCell BiologyBiologyFluorescencePathology and Forensic MedicineCell biologyFlow cytometryGreen fluorescent proteinStainingCytosolCytokineBiochemistryembryonic structuresmedicineNuclear proteinTranscription factor

description

Staining of transcription factors (TFs) together with retention of fluorescent reporter proteins is hindered by loss of fluorescence using current available methods. In this study, it is shown that current TF staining protocols do not destroy fluorescent proteins (FPs) but rather that fixation is not sufficient to retain FPs in the cytosol of the permeabilized cells. In this article, a simple and reliable protocol is elaborated, which allows efficient TF and cytokine staining while retaining FPs inside fixed cells.

yearjournalcountryeditionlanguage
2014-02-19Cytometry Part A
https://doi.org/10.1002/cyto.a.22451