Dažādu kultivēšanas apstākļu ietekme uz Lactobacillus plantarum rezistenci pret oksidatīvo stresu

6533b7d0fe1ef96bd125b1ee

RESEARCH PRODUCT

Dažādu kultivēšanas apstākļu ietekme uz Lactobacillus plantarum rezistenci pret oksidatīvo stresu

Kristīne Kalneniece

subject

Pienskābās baktērijas jonizējošs starojums ūdeņraža peroksīds Bioloģija hinona reduktāze Lactobacillus plantarum

description

Pienskābo baktēriju rezistence pret oksidatīvo stresu, kas cieši atkarīga no šūnu metabolisma, ir ļoti svarīga, lai tās varētu izmantot kā probiotikas. Darba mērķis bija novērtēt oksidatīvā stresa ietekmi uz L. plantarum kultūras enzimātisko aktivitāti pēc tās kultivēšanas dažādos apstākļos un aizsargvielu klātbūtnē. Oksidatīvā stresa ierosināšanai izmantots 1-2 mM H2O2 un 2-50 Gy jonizējošs starojums. Aizsargvielas – sulforafāns (SFA), sulforafēns (SFE). Salīdzinātas De Man, Rogosa and Sharpe (MRS) un Tryptone Soya Broth (TSB) barotnes. Barotnes sastāvs ietekmēja baktēriju augšanas kinētikas parametrus un reakciju uz oksidatīvo stresu. 1 μg/ml SFA vai SFE pievienošana kultūrām pirms apstarošanas radīja ievērojamu ekstracelulāro (P<0.05) hinona reduktāzes un dehidrogenāzes aktivitāšu palielināšanos variantos ar TSB pēc 10 Gy apstarošanas un ar MRS pēc 2 Gy apstarošanas. Darbs izstrādāts LU Mikrobioloģijas un biotehnoloģijas institūtā, laika posmā no 2018. gada septembra līdz 2019. gada maijam. Rezultāti apkopoti 2 zinātniskos rakstos, recenzētos zinātniskos žurnālos. Literatūras apskatā tika izmantoti 59 literatūras avoti. Literatūras dati, metodes un rezultāti tika apkopoti 2 tabulās, 12 attēlos un 6 pielikumos.

year	journal	country	edition	language
2020-01-01

https://dspace.lu.lv/dspace/handle/7/50006

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