0000000000129899
AUTHOR
Kristīne Kalneniece
Dažādu kultivēšanas apstākļu ietekme uz Lactobacillus plantarum rezistenci pret oksidatīvo stresu
Pienskābo baktēriju rezistence pret oksidatīvo stresu, kas cieši atkarīga no šūnu metabolisma, ir ļoti svarīga, lai tās varētu izmantot kā probiotikas. Darba mērķis bija novērtēt oksidatīvā stresa ietekmi uz L. plantarum kultūras enzimātisko aktivitāti pēc tās kultivēšanas dažādos apstākļos un aizsargvielu klātbūtnē. Oksidatīvā stresa ierosināšanai izmantots 1-2 mM H2O2 un 2-50 Gy jonizējošs starojums. Aizsargvielas – sulforafāns (SFA), sulforafēns (SFE). Salīdzinātas De Man, Rogosa and Sharpe (MRS) un Tryptone Soya Broth (TSB) barotnes. Barotnes sastāvs ietekmēja baktēriju augšanas kinētikas parametrus un reakciju uz oksidatīvo stresu. 1 μg/ml SFA vai SFE pievienošana kultūrām pirms apstar…
Effect of Isothiocyanates on the Activity of <i>Lactobacillus plantarum</i> Exposed to Irradiation
Two isothiocyanates, i.e., sulforaphane (SFA) and sulforaphene (SFE), are suggested to be used as an alternative chemopreventive diet. This study was focused on the effect of SFA and SFE on Lactobacillus plantarum, which has been subjected to the irradiation (2-50 Gy). The cultures grown in De Man, Rogosa and Sharpe (MRS) and Tryptone Soya Broth (TSB) were compared in terms of bacteria physiological activity under tested conditions. Broth composition notably influenced the bacteria growth kinetic parameters, as well as culture response to the oxidative stress. Activity of L. plantarum cells after irradiation was evaluated by their dehydrogenase (DHA) and quinone-reductase (QR) activities. T…
Evaluation of Glyphosate Ecotoxicity and Biodegradability in the Municipal Wastewaters
Glyphosate (G) is a broad-spectrum systemic organophosphate herbicide being widely used to control weeds in agricultural fields and urban areas. Its safety for both human health and aquatic biomes is a subject of wide debate. This study was aimed at evaluating the removal efficiency and ecotoxicity of G based herbicide (GBH) Klinik® (Nufarm, Austria) added to the raw municipal wastewater (WW) in a lab-scale model column system. The effect of oxide ceramics as a filtering medium (treatment „B”), as well as activated sludge and nutrients (treatment „C”) was compared with the control columns, which contained only WW (treatment „A”). After 72h treatment of WW spiked with 100 mg/L G, the lowest …