6533b7d0fe1ef96bd125b603
RESEARCH PRODUCT
Quantitative measurements of non-covalent interactions with diamond based magnetic imaging
Metin KayciSean LouretteDmitry BudkerDmitry BudkerTao WangLykourgos Bougassubject
chemistry.chemical_classificationMaterials sciencePhysics and Astronomy (miscellaneous)DiamondNanotechnology02 engineering and technologyengineering.material021001 nanoscience & nanotechnology01 natural sciencesSignalFluorescenceMagnetic fieldMicroelectrodeElectrophoresischemistry0103 physical sciencesengineeringNon-covalent interactions010306 general physics0210 nano-technologyhuman activitiesImage resolutiondescription
We present a technique employing dielectrophoretic (DEP) manipulation of surface immobilized complexes integrated with a magnetic imaging platform based on nitrogen-vacancy (NV) centers in diamond for the quantitative measurements of non-covalent interactions. The interdigitated microelectrodes closely spaced to the functionalized surface of the diamond plate provide a wide range of applied DEP forces for noninvasive manipulation of various molecular interactions, while the NV layer under the surface reports the unbinding dynamics. Given that biological samples do not present significant magnetic background and do not screen magnetic fields, our approach has many advantages over the fluorescent tagging where the optical signal is subject to photo-bleaching, auto-fluorescence, and instabilities. The high sensitivity and spatial resolution provided by NV-based magnetic imaging make this technique a useful tool for biophysical applications.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-07-30 | Applied Physics Letters |