6533b7d0fe1ef96bd125b6e6
RESEARCH PRODUCT
Extractive Spectrophotometric Determination of Ondansetron by Ion-Pair Formation with Bromocresol Green
L. Lahuerta ZamoraJ. Martínez Calatayudsubject
ChromatographyChloroformBromocresol greenmedicine.diagnostic_testCalibration curveBiochemistry (medical)Clinical BiochemistryExtraction (chemistry)Ion pairsBiochemistryAnalytical ChemistryOndansetronchemistry.chemical_compoundchemistrySpectrophotometryElectrochemistrymedicineQuantitative analysis (chemistry)Spectroscopymedicine.drugdescription
Abstract An empirical spectrophotometric procedure for the determination of the antiemetic ondansetron is carried out. The method is based on the formation of a 1:1 ion pair with bromocresol green in the pH range over 3.2 – 4.4, extraction into chloroform layer and spectrophotometric measurement at 420.8 nm. The calibration graph is linear over the range 0.1 – 20 μg ml−1 ondansetron, with a relative standard deviation of 2.7%; the influence of foreign substances is also studied. The method is applied to ondansetron determination in human urine.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-03-01 | Analytical Letters |