6533b7d0fe1ef96bd125b781

RESEARCH PRODUCT

Ruthenium red staining of polyanion containing structures in sections from epoxy-resin embedded tissues

A. TatoA. TatoJ. M. FerrerJ. M. FerrerMaría Gregoria Gutierrez-gonzalvezMaría Gregoria Gutierrez-gonzalvezJuan Carlos StockertJuan Carlos Stockert

subject

MaleIn situRuthenium redHistologyStaining techniqueRutheniumSalivary GlandsMicechemistry.chemical_compoundTongueBone MarrowTestisAnimalsIntestine LargeGlycosaminoglycansStaining and LabelingEpoxy ResinsUterusResolution (electron density)Cell BiologyGeneral MedicineEpoxyRuthenium RedRatsStainingMicroscopy ElectronBiochemistrychemistrySpectrophotometryBORATE BUFFERvisual_artvisual_art.visual_art_mediumDrosophilaFemaleAbsorption (chemistry)Nuclear chemistry

description

Summary Staining by ruthenium red (0.5 mg/ml in borate buffer at pH = 9.2) has been used for light and electron microscopic visualization of polyanion containing structures in sections from glutaraldehyde-fixed, epoxy-embedded tissues. This staining technique can be applied in a simple and rapid way, showing the reactive cell components with suitable resolution and contrast. Preliminary spectrophotometric studies show the correspondence in absorption characteristics of the dye which is bound to polyanions in situ or in vitro .

yearjournalcountryeditionlanguage
1984-01-01Acta Histochemica
https://doi.org/10.1016/s0065-1281(84)80038-0