6533b7d1fe1ef96bd125bae7

RESEARCH PRODUCT

Cytosolic Ca2+Content and Membrane Fluidity of Platelets and Polymorphonuclear Leucocytes in Diabetes Mellitus

Baldassare CaninoAntonio SarnoMaria MontanaAnna CataniaGregorio CaimiR. Lo PrestiG. Ventimiglia

subject

AdultBlood Plateletsmedicine.medical_specialtyAdolescentMembrane FluidityNeutrophilsEndocrinology Diabetes and MetabolismMetabolic aspectsClinical BiochemistryBiochemistryCytosolEndocrinologyDiabetes mellitusInternal medicineDiabetes MellitusmedicineMembrane fluidityHumansPlateletAgedFluorescent DyesChemistryBiochemistry (medical)General MedicineMiddle Agedmedicine.diseasePLATELET MEMBRANE FLUIDITYCytosolDiabetes Mellitus Type 1EndocrinologyDiabetes Mellitus Type 2CalciumFluorescence anisotropy

description

Considering the role played by platelets and leucocytes in diabetic disease and keeping in mind the strong correlation between functional and metabolic aspects that characterizes this clinical condition, we evaluated, in two groups of diabetics, respectively the platelet and polymorphonuclear (PMN) cytosolic Ca2+ content (employing the fluorescent probe Fura 2-AM) and membrane fluidity (using the fluorescent probe TMA-DPH and considering the fluorescence polarization degree, inversely related to the membrane fluidity). From the obtained results, it is evident that the platelet cytosolic Ca2+ content does not distinguish normals from diabetics of type 1 and 2; the platelet membrane fluidity instead does not discriminate normals from diabetics, but differentiates diabetics of type 1 and 2 (type 1 = 0.284 +/- 0.015; type 2 = 0.314 +/- 0.018; p < 0.001). PMN cytosolic Ca2+ content and membrane fluidity do not discriminate normals from diabetics. In the two groups of diabetics none of the platelet and PMN parameters (cytosolic Ca2+ content and membrane fluidity) are related to the glycometabolic pattern.

yearjournalcountryeditionlanguage
1995-08-01Hormone and Metabolic Research
https://doi.org/10.1055/s-2007-979977