6533b7d1fe1ef96bd125c176
RESEARCH PRODUCT
Ontogenetic variations of some enzymes indicentrarchus labrax(Serranidae)
Andrea SantulliVincenzo D'amelioFulvia BasagliaG. Salvatorellisubject
chemistry.chemical_classificationbiologyDehydrogenasebiology.organism_classificationIsozymeMolecular biologyMalate dehydrogenasechemistry.chemical_compoundEnzymechemistryBiochemistryLactate dehydrogenaseGene expressionbiology.proteinAnimal Science and ZoologyDicentrarchusAlcohol dehydrogenasedescription
Abstract The ontogenesis of isozyme patterns of alcohol dehydrogenase (ADH), glycerol‐3‐phosphate dehydrogenase (α‐GPDH), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose‐6‐phosphate dehydrogenase (G‐6‐PD) and glucosephosphate isomer‐ase (GPI) in Dicentrarchus labrax was studied. ADH is active only in the liver of the adult; a‐GPDH is active in only two tissues in the adult: the A2 isozyme in white skeletal muscle and the B2 isozyme in the liver. Differential gene expression was found only for LDH, MDH and GPI, which have polymeric structure. The LDH, MDH and GPI isozymes 30 days after hatching were completely active and showed patterns very similar to those of the adult. Spatial and diachronic variations were related to genetic regulation of expression. Tissue specificity and time of expression during development represent tests of cellular differentiation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1989-01-01 | Bolletino di zoologia |