TLR4 down-regulation identifies high risk HPV infection and integration in head and neck squamous cell carcinomas

6533b7d1fe1ef96bd125c1d8

RESEARCH PRODUCT

TLR4 down-regulation identifies high risk HPV infection and integration in head and neck squamous cell carcinomas

Lorenzo Lo Muzio Giuseppe Colella Corrado Rubini Rosalia Leonardi Mirella Pace Massimo Mascolo Silvia Lepore Iole Natalicchio Giuseppina Campisi Angela Santoro Ilaria Laurenzana Francesco Merolla Eduardo Bucci Giuseppe Russo Gabriella Aquino Renato Franco Vito Rodolico Pantaleo Bufo Giuseppe Pannone Gennaro Ilardi Stefania Trino Bucci P

subject

Oncology 0301 basic medicine Adult Male medicine.medical_specialty Virus Integration Cell Down-Regulation In situ hybridization Biology Alphapapillomavirus General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Retrospective Studie Internal medicine Carcinoma medicine Humans Virus Integration In Situ Hybridization Retrospective Studies Aged Aged 80 and over Innate immune system General Immunology and Microbiology Head and Neck Neoplasm Alphapapillomaviru Expression index HPV infection virus diseases Middle Aged medicine.disease female genital diseases and pregnancy complications Toll-Like Receptor 4 medicine.anatomical_structure 030104 developmental biology Head and Neck Neoplasms Immunology DNA Viral Carcinoma Squamous Cell Immunohistochemistry Female Human

description

TLRs are main actors of the innate immune response against HPV. There are very few studies on the role of TLRs mediated HPV clearance in Head and Neck oncology. Our aim was to evaluate whether TLR4 expression identifies HPV infection and/or HR-HPV integration status in oral and oropharyngeal cancers. By immunohistochemistry we assessed TLR4 levels in OSCC/OPSCC. To detect viral integration or episomic status In situ hybridization for HPV-DNA and Pyro-sequencing techniques have been performed. The relationship between TLR4 expression with HPV infection status has been investigated. ISH HPV positive samples have reported lower levels of TLR4 intensity than negative samples (p = .002). There was no statistical correlation between TLR4 intensity and PCR HPV results (p more than 0.0.5). Point-biserial correlation coefficient revealed significant association between TLR4 expression and HR-HPV integration status (p = .0001) and between TLR4 expression index and HR-HPV infection (p = .001). These data have shown that TLR4 down-regulation is strongly associated to both HPV-16 infection and its integration into the host DNA.

year	journal	country	edition	language
2015-12-29

http://hdl.handle.net/10447/225880