Modulating effects of humic acids on genotoxicity induced by water disinfectants in Cyprinus carpio

6533b7d1fe1ef96bd125c290

RESEARCH PRODUCT

Modulating effects of humic acids on genotoxicity induced by water disinfectants in Cyprinus carpio

Lorenzo Tancioni Marco Rizzoni Carlo Rossi Annamaria Buschini Monica Monfrinotti Paola Poli Bianca Gustavino

subject

Carps Erythrocytes Drinking-water disinfectants Sodium Hypochlorite Health Toxicology and Mutagenesis Humic acids chemistry.chemical_element medicine.disease_cause Water Purification Fish erythrocytes Toxicology chemistry.chemical_compound Micronucleus test Peracetic acid polycyclic compounds Genetics Chlorine medicine Animals Humic acid Peracetic Acid Food science Comet assay Humic Substances chemistry.chemical_classification Chlorine dioxide Micronucleus Tests Comet assay; Drinking-water disinfectants; Fish erythrocytes; Humic acids; Micronucleus test Oxides Comet assay Settore BIO/18 - Genetica chemistry Sodium hypochlorite Micronucleus test Chlorine Compounds Genotoxicity DNA Damage Disinfectants

description

The use of chlorinated disinfectants during drinking-water production has been shown to generate halogenated compounds as a result of interactions of humic acids with chlorine. Such chlorinated by-products have been shown to induce genotoxic effects and consumption of chlorinated drinking-water has been correlated with increased risk for cancer induction in human populations. The aim of this work was to test the potential genotoxic effects on circulating erythrocytes of the fish Cyprinus carpio exposed in vivo to well-waters disinfected with sodium hypochlorite (NaClO), chlorine dioxide (ClO2) or peracetic acid (CH3COO2H, PAA), in the absence or presence of standard humic acids (HA). The effects were measured by use of the micronucleus (MN) and the single-cell gel electrophoresis (Comet) assays at different sampling times after a 3-day exposure period. The exposure to chlorine disinfectants without the addition of HA produced a clear toxic effect. Significant cytogenetic damage (i.e. MN induction) was detected in fish populations exposed to both NaClO and ClO2 with humic acids. In the Comet assay, a significant decrease of DNA migration was observed in erythrocytes of specimens after exposure to NaClO-disinfected water without HA. No effects were observed in any other experimental condition.

year	journal	country	edition	language
2005-11-01	Mutation Research/Genetic Toxicology and Environmental Mutagenesis

https://doi.org/10.1016/j.mrgentox.2005.08.009