6533b7d1fe1ef96bd125c2a5

RESEARCH PRODUCT

Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns

Guillermo Ramis-ramosJosé Manuel Herrero-martínezCecilia B. CastellsLilian Mónica RomeroAgustín Acquaviva

subject

chemistry.chemical_classificationgeographyReproducibilityMonolithic HPLC columnQuenching (fluorescence)Chromatographygeography.geographical_feature_categoryResolution (mass spectrometry)General Chemical EngineeringGeneral EngineeringCiencias QuímicasHigh-performance liquid chromatographyMonolithic ColumnsAnalytical ChemistryAmino acidCore-Shell Columnschemistry.chemical_compoundchemistryCitrullineQuímica AnalíticaMonolithAmino AcidsDerivatizationCIENCIAS NATURALES Y EXACTAS

description

We describe the development of a high-performance liquid chromatography (HPLC) method for the determination of citrulline and other amino acids relevant to intestinal diseases. The amino acids were derivatized with 9-fluorenylmethylchloroformate (FMOC-Cl) and their derivatives were separated on two different columns, a core-shell column (Halo C18) and a silica-based monolith (Chromolith Performance RP-18). The derivatization reaction was optimized with respect to pH, buffer concentration and reproducibility. The optimal derivatization conditions were achieved with 0.4 M borate buffer at pH 9.20, a constant ratio of FMOC-Cl/total amino acids (10 : 1) and 75 mM tyramine after 1 min (quenching reaction). The separation conditions with both chromatographic supports were also optimized. The chromatographic performance (peak capacity and global resolution) of these two columns was compared. This proposed HPLC-UV method was satisfactorily applied to the analysis of a real plasma sample. © 2014 The Royal Society of Chemistry. Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romero, Lilian. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina Fil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina Fil: Ramis Ramos, Guillermo. Universidad de Valencia; España Fil: Herrero Martinez, José Manuel. Universidad de Valencia; España

yearjournalcountryeditionlanguage
2014-08-01
10.1039/c4ay00496ehttp://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/C4AY00496E#!divAbstract