6533b7d1fe1ef96bd125d7ca
RESEARCH PRODUCT
Multiplex PCR Assay for Detection of Vibrio vulnificus Biotype 2 and Simultaneous Discrimination of Serovar E Strains
Carmen AmaroEva Sanjuánsubject
DNA BacterialSerotypeGenotypeVibrio vulnificusPolymerase Chain ReactionApplied Microbiology and Biotechnologylaw.inventionMicrobiologylawVibrionaceaeVibrio InfectionsMultiplex polymerase chain reactionGenotypeAnimalsHumansMultiplexVibrio vulnificusPolymerase chain reactionBacteriological TechniquesEcologybiologybiology.organism_classificationVirologyVibrio InfectionsFood MicrobiologyFood ScienceBiotechnologydescription
ABSTRACT In the present work we develop a multiplex PCR assay for the detection and identification of the fish pathogen Vibrio vulnificus biotype 2 with discriminating potential for zoonotic strains (serovar E). The PCR assay allowed the identification of two new biotype 2 serovar E human isolates from culture collections. Finally, the multiplex was successfully applied to both diagnosis and carrier detection in field samples.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-03-15 | Applied and Environmental Microbiology |