Search results for "Multiplex"
showing 10 items of 337 documents
Performance analysis of Alamouti-coded OFDM systems over spatio-temporally correlated underwater acoustic channels
2017
In this paper, we analyze the performance of Alamouti-coded orthogonal frequency division multiplexing (OFDM) systems over time-varying underwater acoustic (UWA) channels. A realistic UWA channel model has been considered, which can be correlated in either time or space or simultaneously in both domains. An exact analytical expression for the bit error probability (BEP) is necessary to analyze accurately the performance of Alamouti-coded OFDM systems over the spatio-temporally correlated UWA channel model. Hence, by using this UWA channel model, an expression has been derived for the BEP of Alamouti-coded OFDM systems assuming that the receiver knows perfectly the channel state information.…
DNA-Based Identification of Larvae Offers Insights into the Elusive Lifestyles of Native Olive Seed Wasps in South Africa
2020
Wild and cultivated olives in the Western Cape of South Africa are direct or indirect hosts to a high diversity of Braconidae and Chalcidoidea wasps. Olive-associated Braconidae are known to parasitise the larvae of Bactrocera oleae (Rossi), and probably also Bactrocera biguttula (Bezzi). The lifestyle of olive-associated Chalcidoidea is not fully understood, and may include phytophagous, parasitoid and hyperparasitoid species. Some chalcids could represent olive seed wasps (OSW), a generic term that designates the seed feeders responsible for losses in commercial olive production. In order to obtain direct DNA-based evidence for the lifestyle of four putative OSW – Eupelmus spermophilus Si…
Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull (Pagophila eburnea)
2011
We report 22 new polymorphic microsatellites for the Ivory gull (Pagophila eburnea), and we describe how they can be efficiently co-amplified using multiplexed polymerase chain reactions. In addition, we report DNA concentration, amplification success, rates of genotyping errors and the number of genotyping repetitions required to obtain reliable data with three types of noninvasive or nondestructive samples: shed feathers collected in colonies, feathers plucked from living individuals and buccal swabs. In two populations from Greenland (n = 21) and Russia (Severnaya Zemlya Archipelago, n = 21), the number of alleles per locus varied between 2 and 17, and expected heterozygosity per populat…
Multi-application Based Network-on-Chip Design for Mesh-of-Tree Topology Using Global Mapping and Reconfigurable Architecture
2019
This paper outlines a multi-application mapping for Mesh-of-Tree (MoT) topology based Network-on-Chip (NoC) design using reconfigurable architecture. A two phase Particle Swarm Optimization (PSO) has been proposed for reconfigurable architecture to minimize the communication cost. In first phase global mapping is done by combining multiple applications and in second phase, reconfiguration is achieved by switching the cores to near by routers using multiplexers. Experimentations have been carried out for several application benchmarks and synthetic applications generated using TGFF tool. The results show significant improvement in terms of communication cost after reconfiguration.
Genetics of Wilson disease and Wilson-like phenotype in a clinical series from eastern Spain.
2019
Wilson's disease (WD) is an autosomal recessive disorder caused by ATP7B mutations. Subjects with only one mutation may show clinical signs and individuals with biallelic changes may remain asymptomatic. We aimed to achieve a conclusive genetic diagnosis for 34 patients clinically diagnosed of WD. Genetic analysis comprised from analysis of exons to WES (whole exome sequencing), including promoter, introns, UTRs (untranslated regions), besides of study of large deletions/duplications by MLPA (multiplex ligation-dependent probe amplification). Biallelic ATP7B mutations were identified in 30 patients, so that four patients were analyzed using WES. Two affected siblings resulted to be compound…
The Amount of Melanin Influences p16 Loss in Spitzoid Melanocytic Lesions: Correlation With CDKN2A Status by FISH and MLPA.
2019
AIMS The risk assessment of spitzoid lesions is one of the most difficult challenges in dermatopathology practice. In this regard, the loss of p16 expression and the homozygous deletion of CDKN2A, have been pointed in the literature as reliable indicators of high risk. However, these findings are poorly reproducible, and the molecular bases underlying the loss of p16 expression remain unclear. We aimed to identify the underlying events causing loss of CDKN2A/p16 in spitzoid tumors. MATERIALS AND METHODS We evaluated the immunohistochemical expression of p16, and the presence of CDKN2A genetic alterations detected through fluorescence in situ hybridization (FISH) and multiplex ligation-depen…
Identification of Candida auris and related species by multiplex PCR based on unique GPI protein‐encoding genes
2020
Background The pathogen Candida auris is rapidly gaining clinical importance because of its resistance to antifungal treatments and its persistence in hospital environments. Early and accurate diagnosis of C. auris infections is crucial, however, the fungus has often been misidentified by commercial systems. Objectives To develop conventional and real-time PCR methods for accurate and rapid identification of C. auris and its discrimination from closely related species by exploiting the uniqueness of certain glycosylphosphatidylinositol-modified protein-encoding genes. Methods Species-specific primers for two unique putative GPI protein-encoding genes per species were designed for C. auris, …
Role of Hypoxia and the Adenosine System in Immune Evasion and Prognosis of Patients with Brain Metastases of Melanoma: A Multiplex Whole Slide Immun…
2020
Simple Summary The introduction of immune-checkpoint inhibitors improved the therapeutic landscape for patients with advanced malignant melanoma. However, many patients, including patients with melanoma brain metastases, do not derive benefit from immune-checkpoint blockade. Hence, biomarkers are needed to identify potential mechanisms of resistance and optimize patient selection. This study aimed to explore the role of hypoxia-mediated immunosuppression within the tumor microenvironment of patients with metastatic melanoma using multiplex immunofluorescence. We analyzed the prognostic relevance of the hypoxia surrogate marker GLUT-1, the adenosine-synthesizing ectoenzymes CD73/CD39, and th…
Easy One-Step Amplification and Labeling Procedure for Copy Number Variation Detection.
2019
Abstract Background The specific characteristics of copy number variations (CNVs) require specific methods of detection and characterization. We developed the Easy One-Step Amplification and Labeling procedure for CNV detection (EOSAL-CNV), a new method based on proportional amplification and labeling of amplicons in 1 PCR. Methods We used tailed primers for specific amplification and a pair of labeling probes (only 1 labeled) for amplification and labeling of all amplicons in just 1 reaction. Products were loaded directly onto a capillary DNA sequencer for fragment sizing and quantification. Data obtained could be analyzed by Microsoft Excel spreadsheet or EOSAL-CNV analysis software. We d…
Epidemiological and Genetic Characterization of Sapovirus in Patients with Acute Gastroenteritis in Valencia (Spain)
2021
Sapovirus is a common cause of acute gastroenteritis in all age groups. Sapovirus infections are seldom investigated in Spain, and its epidemiology in the country is not well known. The use of molecular diagnostic procedures has allowed a more frequent detection of sapoviruses in patients with diarrhea. A total of 2545 stool samples from patients with acute gastroenteritis attended from June 2018 to February 2020 at the Clinic University Hospital in Valencia, Spain, were analyzed by reverse transcription (RT) and real-time multiplex PCR (RT-PCR) to investigate the etiology of enteric infections. Sapovirus was the second enteric virus detected with a positive rate of 8%, behind norovirus (12…