6533b7d2fe1ef96bd125e0f6

RESEARCH PRODUCT

Corynebacterium aquatimens sp. nov., a lipophilic Corynebacterium isolated from blood cultures of a patient with bacteremia

M. Aravena-romanTimothy J. J. InglisC SieringA. F. YassinCathrin SpröerPeter Schumann

subject

AdultDNA BacterialMaleMolecular Sequence DataCorynebacteriumBacteremiaCorynebacteriumPolymerase Chain ReactionRibotypingApplied Microbiology and BiotechnologyMicrobiologylaw.inventionMycolic acidMicrobiologyRibotypingBacterial ProteinslawRNA Ribosomal 16SHumansPhylogenyEcology Evolution Behavior and SystematicsPolymerase chain reactionchemistry.chemical_classificationBase CompositionCorynebacterium InfectionsbiologyDNA–DNA hybridizationRibosomal RNAbiology.organism_classification16S ribosomal RNArpoBMolecular biologyMycolic AcidschemistrySpectrometry Mass Matrix-Assisted Laser Desorption-Ionization

description

An unknown lipophilic coryneform bacterium isolated from the blood cultures of a patient with bacteremia was characterized by phenotypic and molecular genetic methods. Chemical analysis revealed the presence of short chain mycolic acids consistent with the genus Corynebacterium. The DNA G+C content was 60.8 mol%. Comparative 16S rRNA gene sequence analysis demonstrated that the isolate represents a new subline within the genus Corynebacterium. The closely phylogenetic relative of the unknown bacterium was found to be C. tuscaniense (97.8% sequence similarity). Partial rpoB gene sequence revealed that strain IMMIB L-2475(T) exhibited 13.5% sequence divergence with C. tuscaniense. The unknown bacterium was distinguished from C. tuscaniense by, DNA-DNA hybridization, cellular fatty acid profiles, MALDI-TOF analyses of cell extracts and biochemical tests. Based on the phylogenetic and phenotypic criteria, it is proposed that this bacterium be classified as new species, Corynebacterium aquatimens sp. nov., and is represented by strain IMMIB L-2475(T) (=DSM 45632(T)=CCUG 61574(T)).

yearjournalcountryeditionlanguage
2012-09-01Systematic and Applied Microbiology
https://doi.org/10.1016/j.syapm.2012.06.008