6533b7d2fe1ef96bd125e1d7
RESEARCH PRODUCT
Eliciting callus culture for production of hepatoprotective flavonoids and phenolics from Sequoia sempervirens (D. Don Endl)
Seham S. El-hawaryTanja SchirmeisterUsama Ramadan AbdelmohsenE M Abd El-kaderG. A. Abdel JaleelMohamed A RabehM A Arafatsubject
chemistry.chemical_classificationLiver injuryTraditional medicine010405 organic chemistryBilirubinOrganic ChemistryFlavonoidfungiAlbuminfood and beveragesPhenylalaninePlant Sciencemedicine.disease01 natural sciencesBiochemistry0104 chemical sciencesAnalytical ChemistryElicitor010404 medicinal & biomolecular chemistrySilver nitratechemistry.chemical_compoundchemistryCallusmedicinedescription
The aim of our study is to estimate the hepatoprotective effects of the ethanolic extract of the leaves of Sequoia sempervirens by determination of liver biomarkers (ALT, AST, total bilirubin and albumin in serum) and by histopathological examinations using thioacetamide-induced (TAA) liver injury model. Concurrent administration of ethanolic extracts of S. sempervirens leaves improved the alterations in liver morphology where it was a potent protector of the liver. The potential of L-phenylalanine and silver nitrate as chemical elicitors as well as UV radiation as a physical elicitor on flavonoid production in callus culture of S. sempervirens were emphasized. Murashige and Skoog’s medium fortified with phenylalanine and silver nitrate enhanced the production of flavonoids and phenolics. HPLC analysis was performed for qualitative and quantitative estimation of some flavonoid compounds in the produced calli in comparison with the mother plant. This finding highlights the potential use of S. sempervirens in the treatment of liver dysfunction.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-01-01 |