6533b7d2fe1ef96bd125e36d
RESEARCH PRODUCT
Spontaneous cytotoxic activity of eosinophilic granule cells separated from the normal peritoneal cavity ofDicentrarchus labrax
Melchiorre CervelloMatteo CammarataNicolò ParrinelloMirella VazzanaVincenzo Arizzasubject
Cytotoxicity ImmunologicPathologymedicine.medical_specialtySettore BIO/05 - ZoologiaCell SeparationAquatic SciencePeritoneal cavitychemistry.chemical_compoundLactate dehydrogenaseCentrifugation Density GradientTumor Cells CulturedmedicineAnimalsHumansEnvironmental ChemistryCytotoxic T cellCytotoxicityPeritoneal CavitybiologyOsmolar ConcentrationGranule (cell biology)Dicentrarchus labrax Teleostei cytotoxicity peritoneal cavity eosinophilic granule cellGeneral MedicineCytotoxicity Tests Immunologicbiology.organism_classificationMolecular biologyEosinophilsmedicine.anatomical_structurechemistryCell cultureBassDicentrarchusRabbitsPercolldescription
Abstract In this study the spontaneous in vitro cytotoxic activity to tumour cell lines, (K562), by unstimulated sea bass ( Dicentrarchus labrax ) leukocytes was examined by trypan blue exclusion test and lactate dehydrogenase release assay. A high anti-tumour cell line activity of resident peritoneal leukocytes was found at an effector to target ratio (E:T) of 25:1 after incubation for 2 h at 18° C. Rabbit and sheep erythrocytes were not lysed. A low activity was displayed by head kidney and spleen cell populations whereas blood leukocytes revealed no significant activity. The effect of E:T ratio on cytotoxicity as well as microscopy observations suggested that the cytotoxic reaction required effector-target cell contact. Eosinophilic granule cells, isolated on a Percoll density gradient from a peritoneal wash, appeared to be responsible for the in vitro cytotoxic activity.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-02-01 | Fish & Shellfish Immunology |