6533b7d2fe1ef96bd125eabe
RESEARCH PRODUCT
Assessment of temperature effects on beta-aggregation of native and glycated albumin by FTIR spectroscopy and PAGE: relations between structural changes and antioxidant properties.
Philippe RondeauSerge ChesneSergio ArmentaHenri CaillensEmmanuel Bourdonsubject
Glycation End Products AdvancedAntioxidantTime FactorsFree Radicalsmedicine.medical_treatmentBiophysicsThermal treatmentProtein aggregationBiochemistryAntioxidantsProtein Structure SecondaryStructure-Activity RelationshipGlycationSpectroscopy Fourier Transform InfraredmedicineAnimalsGlycated Serum AlbuminBovine serum albuminFourier transform infrared spectroscopyMolecular BiologyPolyacrylamide gel electrophoresisSerum AlbuminChromatographyBinding SitesbiologyChemistryAlbuminTemperatureSerum Albumin Bovinebiology.proteinCattleElectrophoresis Polyacrylamide GelCopperdescription
Abstract Structural modifications of bovine serum albumin (BSA) induced by heating, and the involvement of glycation of albumin in such processing were studied by using Fourier transform infrared spectroscopy (FTIR) and polyacrylamide gel electrophoresis (PAGE). For native BSA, heating treatments gave rise to β structures which were amplified to the detriment of α-helix form, and which were associated with increased aggregation. A very high correlation was obtained between FTIR Amide I band evolution and aggregation rate parameters, showing the contribution of β-form in aggregates formation. We further assessed the effect of glycation on protein sensibility to heating treatments. A reduction of conformational changes and aggregation processes was demonstrated for the glycated form of the protein. The antioxidant properties of albumin were evaluated using two different techniques assessing metal binding and free radical neutralizing capacities of the protein. Associations between structural changes in BSA induced by the thermal treatment and its antioxidant activities were established.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-04-01 | Archives of biochemistry and biophysics |